• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Lipid-Mediated Gene Transfer into Normal Adult Human Hepatocytes in Primary Culture

        互联网

        525
        Functional analysis of the 5′-flanking region of a gene has become a routme procedure for identifying the cis -acting DNA elements involved in the control of the transcriptional activity of the gene (1 ). This experimental approach requires the transfection of reporter plasmids, harboring various fragments of the 5′-flanking region of the gene, into appropriate cultured cells and the subsequent measurement of the activity of the reporter gene, either constitutively or in response to regulatory stimuli. Continuous cell lines derived from mahgnant tissues have been extensively used for this purpose because they can be easily cultured and transfected. However, these cell lines have two major drawbacks: they are dedifferentiated with respect to the normal tissue from which they originate; that is, the tissue-specific transcription factors are generally expressed at a low level, if at all; and their culture requires the use of serum, the chemical and hormonal composition of which is not fully defined. In fact, it would be preferable to transfect normal cells in a primary culture in which the phenotype of the tissue of origin is maintained. The problem with this approach is that the methods currently used to transfect cell lines, including electroporation and calcmm phosphate-or diethylaminoethyl (DEAE) dextran-DNA coprecipitation, are generally not convenient for primary cultures because of their toxicity (2 4 ).
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序