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        G-Protein-Coupled Receptor Phosphorylation and Palmitoylation

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        It is now clear that nearly all G-protein-coupled receptors (GPCRs) are phosphorylated and palmitolyated. The process of receptor phosphorylation has been extensively studied because it offers a regulatory mechanism that is both rapid and dynamic. However, it has recently become clear that palmitoyaltion of GPCRs at C-terminal cysteine residues may also offer dynamic receptor modification. A growing number of GPCRs have been demonstrated to undergo rapid agonist-mediated changes in their palmitoylation status with functional implications to receptor signaling. This chapter aims to outline the methods we have used to investigate agonist-mediated changes in GPCR phosphorylation and palmitoylation.
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