• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Studies of Local Bone Remodeling: The Calvarial Injection Assay

        互联网

        216
        There are several assays available to study the effects of cytokines, drugs, and hormones on bone cells in vitro. However, as the complex interactions between cells are disrupted, these in vitro assays do not always reflect what happens in vivo. The calvarial injection method, originally described by Boyce et al. (1 ), is valuable for studying the effects of substances on bone metabolism in vivo. In this assay, the substance to be tested is injected subcutaneously over the calvarium of a mouse. At the end of the assay the animal is euthanized, and the calvarium dissected and analyzed by microscopy. Although the assay was originally used to study effects of cytokines on osteoclast formation and activity (1 ,2 ), it has also been used to study the effects of drugs on bone formation (3 ).
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序