Isolation of Mitochondria, Mitochondrial Membranes, Lysosomes, Peroxisomes, and Golgi Membranes from Rat Liver
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This fraction is defined broadly as the material that will sediment at about 3500g for 10 min from a postnuclear supernatant. It contains the nuclei that failed to sediment at 1000g for 5–10 min, the largest mitochondria, and very few other organelles, such as lysosomes and peroxisomes.
