Expression of Eukaryotic Cytochromes P450 in E. coli

Although much has been learned from the study of relatively abundant cytochromes P450 (e.g., P450_cam from Pseudomonas putida cytosol, P4501A2 and P4502B4 from rabbit liver microsomes, and P45Oscc from bovine adrenal cortex mitochondria), the development of cytochrome P450 heterologous expression systems (l – 3 ), especially in Escherzchza coli (4 ), has provided an opportunity to address questions relating to the structure/function of less abundance dant forms of cytochrome P450 whose cDNAs (CYP cDNAs) have been isolated by recombinant DNA technologies. Among the advances achieved