Carbonate Solution For Western Blot
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CARBONATE WESTERN TRANSFER SOLUTIONS
Dunn, Anal. Biochem. 1986: 157
Dunn, Anal. Biochem. 1986: 157
| 1.5L | 1.0L |
||
| 10mM NaHCO3 | 1.26g | chill to 4oC | 0.84g |
| 3mM Na2CO3 | 0.477g | pH ~9.9 | 0.318g |
| in 20% MeOH | 300mL | 200mL |
1. Blot: no need to presoak gell.
Transblot: 1-2H @ 1A keep T<35oC
Miniblot: 1H @ 250mA
2. Assemble wet sandwich on black side:
black side holder
brillo pad
3MM paper
gel
nitrocellulose
3MM
brillo
white side holder
