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Detection of Hepatitis B Virus X Antigen by Immunohistochemistry and Western Blotting

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The genome of hepatitis B virus (HBV) is a partially double-stranded DNA molecule within virus particles that is approx 3.2 kbp long (1 ,2 ). It has four open reading frames, all on one strand, that encode the surface antigen or envelope polypeptides, core or nucleocapsid polypeptide, the viral polymerase, and the so-called “X” protein (1 ,2 ).
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