High-Throughput Expression and Purification of 6xHis-Tagged Proteins in a 96-Well Format
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732
By using automation and affinity-tag technologies, analysis of the large number of ORFs generated by genome-sequencing projects is greatly accelerated. Protocols describing culture of E. coli in automation-compatible formats and subsequent microto large-scale automated purification of 6xHis-tagged proteins are presented.
