• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Quantitation of mRNA Species by RT-PCR on Total mRNA Population with Nonradioactive Probes

        互联网

        528
        Quantitative polymerase chain reaction (PCR) is aimed to determine the absolute or relative amounts of RNA or DNA sequences in a given sample. There are two facts limiting the convenience of this approach. First, in most cases, only one or two sequences are amplified in a given round of amplification. If a family of sequences are to be quantitated, as many amplification reactions are necessary. However, it has been shown that complex populations could be amplified in a sequential independent way (1 3 ). A major concern about the amplification of whole populations are the biases for or against some sequences. In fact, it appears that these biases are not important and that the amplified populations are quite representative of the original mixture of sequences (1 ,4 ). This makes possible a score of PCR applications such as differential display analysis (5 ) or representational difference analysis (6 ), which are aimed to detect qualitative and quantitative differences between sequences present in genomes or messenger RNA (mRNA) populations. This also implies that it is possible to measure the amount of numerous sequences in the amplicons.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序