Cloning into M13

The bacteriophage M13 has been developed into a cloning vector system for obtaining single-stranded DNA template required for the dideoxy chain termination method of sequencing DNA (1 ,2 ). General aspects of bacteriophage M13 as a cloning vector system are reviewed in Chapter 2 , and the preparation of foreign DNA fragments for M13 cloning is described in Chapter 7 . In this chapter the preparation of M13 vectors and the ligation of foreign DNA fragments (inserts) into M13 vectors are described.