M13 and Phagemid-Based Cloning Vectors
互联网
575
Bacteriophage-based vectors, such as λ have been useful in creating gene llbrarles and in the expression of proteins However,
a number of techniques used in molecular biology require DNA to be ina single-stranded form, such as chain termmatlon sequencing,
ollgonucleotlde-directed mutagenesis, and certain probe synthesis strategies A small number of naturally occurring viruses
produce single-stranded DNA as part of their life cycle, these include M13, fl , and fd, and a number of them have been mampulated
and adapted as cloning vectors for the purpose of single-strand DNA production