• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Experimental Identification of MicroRNA Targets by Immunoprecipitation of Argonaute Protein Complexes

        互联网

        533
        MicroRNAs (miRNAs) represent a class of small noncoding RNAs that negatively regulate gene expression�. Intensive research during the past decade has established miRNAs as key regulators of many cellular pathways. MiRNAs have also been implicated in a number of diseases including various forms of cancer. Mammalian miRNAs associate with members of the Argonaute (Ago) protein family and function in multi-protein complexes. MiRNAs guide Ago protein complexes to partially complementary sequences typically located in the 3′ untranslated region (UTR) of their target mRNAs leading to the inhibition of its translation and/or its destabilization. To understand the biological roles of miRNAs, it is essential to identify the mRNA targets that they regulate. Because of the low degree of complementarity between the miRNA and its target sequence, it is often difficult to find targets computationally. Therefore, biochemical methods are needed to identify miRNA targets experimentally. The availability of highly specific monoclonal antibodies against Argonaute proteins allows for the isolation of functional Ago-miRNA–mRNA complexes from �different cell lines, tissues, or even patient samples. Here we provide a detailed protocol for isolation and identification of miRNA target mRNAs from immunoprecipitated human Ago protein complexes.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序