Neutralization of HIV-1 Infection of Human Peripheral Blood Mononuclear Cells (PBMC): Antibody Dilution Method

Antibodies elicited by passive or active immunization protect against numerous virus diseases. This in vivo protective immunity is often associated with in vitro detection of neutralizing antibodies (NAb) (e.g., polio, measles, influenza, respiratory syncytial virus, yellow fever, dengue, rabies, varicella, hepatitis A, hepatitis B) (1 –7 ). Since human protection from HIV-1-associated disease has not yet been achieved, the role of NAb in protective immunity is not defined. Nonetheless, based on experience with other viruses, it is reasonable to assume that NAb will play an important role in protection against HIV-1.