DNA转染

 

DNA 转染

 

・         Transfection of Mammalian Cells Using Lipofectamine (LTI)

・         Guide to Eukaryotic Transfections with Cationic Lipid Reagents (PDF) (LTI)

・         Transfection of Mammalian Cell (Calcium Phophate Method) (Botwell Lab)

・         Transfection of Mammalian Cell (Electroporation Method) (Botwell Lab)

• Transfection of Mammalian Cells by the Calcium-Phosphate Method (Tyner Lab)
  For transient or stable transfection
        

・         Transient or Stable Transfection of HeLa Cells (LTI)
Procedure for Transient or Stable Transfection of HeLa Cells Procedure for Transient or Stable Transfection of HeLa Cells In a six-well or 35 mm tissue culture plate, seed ~4 x 10^5 cells per well...

・         Transfection of Mammalian cell (Life Technologies)
Procedure for Transient or Stable Transfection

・         Eukaryotic Cell Transfection (PMCI Research)

o        Soft Agar Assay for Colony Formation #1

o        Soft Agarose Assay #2

o        Virus Infection of Fibroblasts

o        DNA Transfection of Eukaryotic Eells Using Calcium Phosphate

o        Lipofectamine for Transformation of Eukaryotic Cells

o        Electroporation of COS-7 Cells

o        Growth and Transfection of PC12 cells

o        ß-Gal Staining of Eukaryotic Cells In Vitro

・         Selection of Transfected Suspension Cells (Hannun/Obeid Lab)

・         CAT Assay (LTI)
Detailed and general protocol

・         CAT assay (Woodgett Lab)

・         CAT Assay (Promega)
Chloramphenicol acetyltransferase (CAT), encoded by a bacterial drug-resistance gene, inactivates chloramphenicol by acetylating the drug at one or both of its two hydroxyl groups (1). This gene is not found in eukaryotes, and therefore eukaryotic cells contain no background of CAT activity.

・         Luciferase assay reagents (Crawford Lab)

・         Beta-Galactosidase Enzyme Assay (Promega)
For assaying beta-galactosidase activity in extracts prepared from cells transfected with beta-galactosidase reporter vectors. 

• ß-gal staining of eukaryotic cells in vitro (Botwell Lab)
  Cells previously transfected with a lac Z construct can be stained for b-galactosidase activity.
    

・         Beta-Galactosidase Assay  
   

・         Assay for ß-Galactosidase Expression in situ (Life Technologies)
Assay for ß-Galactosidase Expression in situ Assay for ß-Galactosidase Expression in situ Transfect cell cultures with expression vector for ß-galactosidase, allow expression for 1-2 days... 
  

・         FAQs for Antibiotics (LTI)
tips for does and preparation of Geneticin (G418)