• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Human Y1/Y5 Receptor Gene Cluster: Isolation and Characterization

        互联网

        478
        The methods used to isolate genomic clones of a particular gene have changed significantly over the years. In particular, the characterization of genes with many exons, separated by large introns, increased the need for clones with large inserts. The average length of an insert contained within one of the classical used λ cloning vectors (λgt10 or EMBL3) ranges from 10 to 15 kb. This insert size might be large enough to contain entire genes that contain no or only a few introns, but it is likely that such clones harbor only parts of larger genes. In many cases several overlapping λ clones must be identified and isolated by repeated screening (“chromosomal walking”) of a library with end fragments isolated from the first clone. This significantly increases the time and effort involved in isolating a gene. In addition, the isolation of λ DNA is a laborious and time-consuming exercise that yields only low amounts of DNA.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序