DNA SampleMatrix Protocol

 

Objective:  Preserve plasmid and genomic DNA.

Storage:

1. Open tube or remove seal from plate
2. Add 1-20μl of DNA to tube or well (from picograms up to 30μg)
3. Dry samples completely at room temperature

   Overnight on the bench-top or in laminar flow hood 
   Faster drying times can be achieved using a vacuum (refer to Biomatrica drying guidelines)

4. Store dried samples in an airtight container at room temperature.

   For optimal results dry samples should be sealed with an ambient humidity of less than
   50%.

Sample Recovery:

1. Add 10-50 μl of water tube or well
2. Hydrate for 15 minutes, mixing not necessary
3. Use directly in downstream application
4. Sample can be re-dried to preserve remaining DNA. Refer to storage steps 2&3.

 

Printable Protocol

 

Drying samples in  Sample Matrix   with a Speedvac

Protocol

Objective:   Preserve plasmid and genomic DNA samples in SampleMatrix by drying
samples down using a Speedvac.

Appropriate drying times:

Volumes to SpeedVac	Recommended Drying Time
10-20μl	30 minutes
>20μl-30μl	1 hour
>30μl-50μl	1 hours
>50μl-100μl	1 hours

~undefinedPlease note that no heat is used while drying down your DNA samples.