实验动物的体重和周龄

Protocol to Count Cell Number of Preimplantation Embryos using Nuclear Staining with Hoechst 33342 or DAPI Introduction The following is a simple procedure for determining cell number in preimplanta ...

ContentsEmbryonic Stem Cell Markers Hematopoietic Stem Cell Markers Mesenchymal/Stromal Stem Cell Markers Neural Stem Cell Markers References While stem cells are best defined functionally a number of ...

In Vitro Production of Bovine Embryos (P.J. Hansen Lab Dept. of Animal Sciences University of Florida)This protocol describe procedures for in vitro production in vitro fertilization (IVF) and in vit ...

Materials8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C � do not go higher). Add a few drops of 2 N sodium hydroxide until ...

1. The basic unit is constructed from a media filtration unit such as Corning’s 115 ml filter system (catalog numbers 430944-430947). 2. Tygon tubing (3.2 mm i.d. x 6.4 mm o.d.) with a stopcock fasten ...

ABSTRACT Knowledge of the geneological relationships of cells during development can allow one to gain insight into when and where developmental decisions are being made. Geneological relationships ca ...

8 eggs per day day 7- day 13 cut CAM wash in precooled PBS in 10 ml WASH 1 (PBS 5 mM EDTA COMPLETE) on ice cut in pieces in petri dish on ice centrifuge 4 min at 1000 rpm (table top centifuge Falcon 1 ...

1. The basic unit is constructed from a media filtration unit such as Corning’s 115 ml filter system (catalog numbers 430944-430947). 2. Tygon tubing (3.2 mm i.d. x 6.4 mm o.d.) with a stopcock fasten ...

1. Sacrifice impregnated mouse.2. Dissect out the uterus of the mouse. Pulling up on the uterus with one set of forcepsuse another to tear the mesometrium away from the uterus.3. Remove the embryos fr ...

Ovulation is induced in X. tropicalis by injection of human chorionic gonadotropin (hCG). X. tropicalis requires a much smaller dose than does X. laevis. We use basically the same protocol as that des ...

In order to get the best in vitro fertilization two criteria appear to be critical: first selection of a mature male and second use of L15/CS (L-15 Medium supplemented to 10% calf serum).Select a mal ...

For natural matings single pairs of boosted males and females are placed together. We usually keep them on a darkened shelf away from disturbances but where they can be easily monitored. Three to four ...

Spawning can be induced in adult sea urchinsby injecting 1 ml of a0.5M KCl solution into several sites in the soft membrane around the mouth. Within minutes the gametes should appear: the sperm are of ...

Gametes are collected as described. To remove some of their jelly coatwash eggs several times in ASW by allowing them to settle and gently pouring off excess liquid. Transfer enough eggs to barely cov ...

Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity1. Obtain embryo samples tube of AP substrate buffer and tube of phosphate buffered saline (PBS) for each grou ...

The following is written to help non-marine biologists use sea urchins occasionally in teaching; disregard if you have running sea water tanks in your lab!Gravid animals can be obtained virtually year ...

Invertebrate embryos develop at different rates depending on the temperature of incubation. Descriptive stages based on the number of cells and their arrangement in the embryo are used to enable resea ...

Objective: to test the effects of inhibiting the fast and slow blocks to polyspermy on sea urchin development. BackgroundSea urchin eggs release components of the egg jelly that attract sperm and indu ...

Purpose This experiment will study effects of lithium chloride on sea urchin development forcusing on archenteron formation. IntroductionGastrulation is extensive cell rearrangement where cells underg ...

The Effects of NiCl2on Spicule FormationJessica Ann Billet Franklin and Marshall Class of 2000Background and ObjectiveSea urchins exhibit radial holoblastic cleavage eventually forming a blastula. Sho ...