• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Mini-Chamber for Regulating Gaseous Environment During Culture

        互联网

        723

         

        1. The basic unit is constructed from a media filtration unit such as Corning ’s 115 ml filter system (catalog numbers 430944-430947).

        2. Tygon tubing (3.2 mm i.d. x 6.4 mm o.d.) with a stopcock fastened to the end is attached to the hose connector.

        3.  A 00 size rubber stopper is inserted into the pour spout of the filter system.

        4. The membrane in the filter system is punctured in several places to facilitate movement of gases.

        5. Culture plates (up to a 60 mm petri dish plate) are gently positioned on top of the membrane. If the plate is large, it is difficult to maneuver the plate into the chamber with fingers.  In this case, a cradle underneath the plate can be constructed with tape to facilitate placement of the dish.

        6. The lid of the filter system is taped securely with scotch tape and parafilm is placed around the lid to make the chamber airtight.

        7. Gases are injected for 3 min through the pour spout from a hose connected to the gas cylinder while the stopcock was in the open position.

        8. After injection of gases is completed, the rubber stopper is fastened to the pour spout and the stopcock is moved to the closed position.

        9. To prevent contamination during injection of gases, a filter (0.22 m m) is attached to the gas hose.

        <center> <p> </p> </center>

         

        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序