DNA重组

Preparation of linear polyacrylamide used as carrier in ethanol precipitation of nucleic acidsLinear polyacrylamide can be used as an efficient neutral carrier for precipitating nucleic acids with eth ...

DNA Extraction MethodsPhenol-chloroform DNA extraction from sand flies1. Crash Individual sand flies in 0.5 ml lysis buffer (50 mM NaCl 10 mM EDTA 50 mM Tris-HCl pH 8). 2. Incubate The sand fly ho ...

DNA Extraction MethodsPhenol-chloroform DNA extraction from sand flies1. Crash Individual sand flies in 0.5 ml lysis buffer (50 mM NaCl 10 mM EDTA 50 mM Tris-HCl pH 8). 2. Incubate The sand fly ho ...

Rapid Method for Preparation of Genomic DNA from P. aeruginosa Inoculate 1 ml of L-broth or other rich media with cells of the strain from which genomic DNA is to be isolated. Grow this culture at 37° ...

Rapid Method for Preparation of Genomic DNA from P. aeruginosa Inoculate 1 ml of L-broth or other rich media with cells of the strain from which genomic DNA is to be isolated. Grow this culture at 37° ...

Preparation of Sonicated Salmon Sperm DNAProcedure:1) Use Pharmacia #27-4564-01. With clean flamed scissors and forceps weigh 0.25 g/50 ml conical and add 50 ml/conical of 0.02 M Tris pH 7.6. Allow to ...

This is a modification of a salting out procedure as described by Miller et al. (1988) evaluated at the DNA Laboratory Medical School Malta. When analysed by spectrophotometry 95% of extracted genomic ...

This is a modification of a salting out procedure as described by Miller et al. (1988) evaluated at the DNA Laboratory Medical School Malta. When analysed by spectrophotometry 95% of extracted genomic ...

DNA From Whole Blood for PCR(from Higuchi R. (1989) Amplifications 2: 1-3)Obtain 65-100 µl of blood by retro-orbital bleed with a heparinized microcapillary tube. Expel blood immediately into a 1.5 ml ...

Extraction of genomic DNA from whole bloodAuthor: Laura-Lee BoodramSource: Laura-Lee Boodram Department of Life Sciences The University of the West IndiesAbstract: The protocol is simple and fairly ra ...

DNA ISOLATION FROM PRIMARY TUMORS VIA CRYOSECTIONS - make a 5µm section to do an evaluation of the % tumour cells- make 50X50µm sections for the DNA isolation- put sections in a falcon tube containing ...

ES CELL DNA EXTRACTION: TUBE METHOD Protocol for extracting DNA from ES Cells starting from the 96-well plate but processing in an eppendorf tube to recover more of the DNA. NOTE- THIS TAKES A LOT OF ...

macerate tissue in Eppendorf tube without butter at RT add 400 ml extraction buffer vortex for 4 sec leave sample at RT until other samples are ready ( 1 h) spin in microfuge for 1 min transfer 300 ml ...

Materials:phenol:chloroform (1:1)chloroformAdd an equal volume of buffer-saturated phenol:chloroform (1:1) to the DNA solution. Mix well. Most DNA solutions can be vortexed for 10 sec except for high ...

Materials:phenol:chloroform (1:1)chloroformAdd an equal volume of buffer-saturated phenol:chloroform (1:1) to the DNA solution. Mix well. Most DNA solutions can be vortexed for 10 sec except for high ...

Simplified DNA Extraction from Cell or TissueAuthor: Long-Cheng LiSource: Protocol OnlineAbstract: This method doesn't require organic extraction and centrifugation. It's the most simplest way of prep ...

DNA and RNA EXTRACTIONSA protocol / method / schedule /procedure for extraction / isolation of both DNA and RNA from the same material typically plant leaf / leaves(See also DNA Isolation protocol)1) ...

DNA and RNA EXTRACTIONSA protocol / method / schedule /procedure for extraction / isolation of both DNA and RNA from the same material typically plant leaf / leaves(See also DNA Isolation protocol)1) ...

Procedure 1. Grind 2 to 5 g of frozen leaves to a very fine powder with a liquid nitrogen-cooled mortar and pestle. 2. Add 25 ml of CTAB Buffer and transfer to a 50 ml tube. 3. Incubate at 65°C for 2 ...

Procedure 1. Grind 2 to 5 g of frozen leaves to a very fine powder with a liquid nitrogen-cooled mortar and pestle. 2. Add 25 ml of CTAB Buffer and transfer to a 50 ml tube. 3. Incubate at 65°C for 2 ...