Phenol/chloroform Extraction of DNA

Materials:

	phenol:chloroform (1:1)
	chloroform

1. Add an equal volume of buffer-saturated phenol:chloroform (1:1) to the DNA solution.
2. Mix well. Most DNA solutions can be vortexed for 10 sec except for high molecular weight DNA which should be gently rocked. (If using Phase-Lock Gel, follow procedure M.1 )
3. Spin in a microfuge for 3 min.
4. Carefully remove the aqueous layer to a new tube, being careful to avoid the interface.
5. (Steps 1-4 can be repeated until an interface is no longer visible)
6. To remove traces of phenol, add an equal volume of chloroform to the aqueous layer.
7. Spin in a microfuge for 3 min.
8. Remove aqueous layer to new tube.
9. Ethanol precipitate the DNA