其他模型

Procedure pick one colony inoculate in 3 ml of the appropriate media grow at 30° overnight pellet the cells (5 min 5000g) wash 1X in sterile ddH2O re- suspend the pellet in 200 µl EthOH (optional: +2 ...

This is the preferred method for yeast RNA preparation use Gloves and RNAse free solutions throughout.1. Use a YPD overnight culture to innoculate fresh YPD media and grow cells at 30 degrees overnigh ...

Day 1Set up an overnight culture in 100 ml LMM broth or 100 ml terrific broth containing 100ul 100 mg/mlAmp Day 2Add 40-50 ml o/n culture to 1 lt terrific/K2K (see appendix) with 0.5 ml 100mg/ml Amp. ...

Inoculate cells from an overnight culture into 50 ml YEPD and incubate at 30°C with shaking. Typically add 0.1 to 0.2 ml saturated culture in the evening to get an of OD600 = 1 to 2 the next morning. ...

Transformation Protocol for Arabidopsis � Abbreviated Germinate seed in pots ↓ 4 weeks Streak bacteria onto YM/MinA ↓ 2-3 days 28°C Spray/dip bacterial suspension onto plants ↓ 1 day in box Let plants ...

This is a simple and fast protocol for the extraction of genomic DNA from Arabidopsis thaliana that works fine in PCR for simple amplicons. We only use this with (rosette) leaves but it is likely that ...

OverviewIn order to study spleen cells (e.g. lymphocytes granulocytes other immune cells) it helps to make single-cell suspensions so that the cells can be manipulated ex vivo easily. This protocol su ...

One of the uses of tamoxifen in the lab is the activation of enzymes deactivated via an estrogen-binding domain like Cre-ERT (Cre-LBD). Tamoxifen is an estrogen analogue that binds with higher affinit ...

PRIMARY MOUSE KERATINOCYTE CULTURESIsolation of epidermal keratinocytes from neonatal mice is based on the protocol of Dlugosz et al. Methods Enzymol. 254:3-20 (1995). The epidermis from a newborn mou ...

Setup You have yeast strains that are deficient in mating (eg Ste12 knockouts) and would like to test whether transforming them with a plasmid that contains genes that are supposed to complement the m ...

MethodUsing sterile pipette tips transfer a 1 mm colony into 50 uL of 60 U/ml Zymolyase 3 uL of 1 U/mL Zymolyase stock solution 47 uL of water Incubate cell suspension at 37 C for 30 minutes. Incubate ...

Materials (Solutions are all available from the media room) 200ml bottle of 2x SD 200ml bottle of 4% agar -- make sure to sign it out 40% glucose CSM minus the relevant marker(s) as powder Stack of pl ...

General Information The cells being plated are sandwiched between layers of cell-free agar. The submerged cells grow into smaller colonies and many more colonies can be counted per plate (I''ve been a ...

Zymolyase Solution: Regular stock of zymolyase is 10 mg/ml diluted in water mix by inverting not all will go into solution (filter it) store aliquots at -20°C (minimize freeze-thawing if possible) Mak ...

INTRODUCTION The Drosophila larval brain is a well-established model for investigating the role of stem cells in development. Neuroblasts (neural stem cells) must be competent to generate many thousan ...

Single-Cell Electroporation in XenopusXue Feng Liu and Kurt Haas INTRODUCTIONSingle-cell electroporation (SCE) is a versatile technique for delivering electrically charged macromolecules including DNA ...

SOS信号转导途径在植物离子平衡和耐盐中非常重要。SOS模型认为高Na+引起了胞内自由Ca2+的升高，激活了Ca2+结合蛋白编码的SOS3的表达，影响到下游的反应。SOS3激活了相连的SOS2（丝氨酸/苏氨酸蛋白激酶），SOS2/SOS3复合体调节盐忍耐因子编码的SOS1（质膜Na+/H+反向转运体）的表达水平，从而促使胞内过多的Na+排出体外。这其中涉及到K+的变化，Na+和K+平衡的破坏可能是 ...

In Situ Hybridization to Somatic Chromosomes in DrosophilaAbby F. Dernburg INTRODUCTIONIn situ hybridization was originally developed as a technique for visualizing and physically mapping specific seq ...

小鼠(T)酶联免疫分析（ELISA）试剂盒使用说明书本试剂仅供研究使用目的：本试剂盒用于测定小鼠血清，血浆及相关液体样本中*~AT~B含量。实验原理：本试剂盒应用双抗体夹心法测定标本中小鼠*~AT~B水平。用纯化的小鼠*~AT~B抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入*~AT~B，再与HRP标记的*~AT~B抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底 ...

小鼠破伤风(Tetanus)酶联免疫分析（ELISA）试剂盒使用说明书本试剂仅供研究使用目的：本试剂盒用于检测小鼠血清，血浆及相关液体样本中破伤风(Tetanus)水平实验原理：本试剂盒采用双抗体夹心酶联免疫法（ELISA）测定标本中小鼠破伤风(Tetanus)。用纯化的小鼠破伤风(Tetanus)抗体包被微孔板，制成固相抗体，可与样品中破伤风(Tetanus)相结合，经洗涤除 ...