疾病及处理

The herpes simplex virus (HSV) lytic cycle is dependent on a precise temporal pattern of viral gene expression with the initial expression of the immediate-early (IE) genes, followed by the early genes, and finally late gene expression (1). Although such a temporal cascade of viral gene express ...

In order to identify the nature of posttranslational modifications and enzyme functions of herpes simplex virus 1 and 2 (HSV-1 and HSV-2) proteins, it is necessary to apply both biochemical and genetic analyses. The experimental methods described in this chapter have been applied to cells cu ...

Animal models remain essential for studies of many aspects of the biology of herpes simplex virus (HSV). Such studies include basic experiments on pathogenesis (including characterization of viral mutants), tests of antiviral drugs, and methods of immunization. With reference to mod ...

Whether herpes simplex virus (HSV) is viewed as a pathogen or as a model eukaryotic system, it is virtually certain that any experimental work will require the virus to be grown and assayed. The following chapter is therefore seen as the fundamental first step before embarking on more intellectua ...

Research on herpesvirus infections has commanded the attention of a diverse group of scientists for over a century. Until the advent of the human immunodeficiency virus, the herpes simplex viruses (HSV) were the most intensively studied of all viruses. During the early part of the nineteenth c ...

We have developed an in vitro model of herpes simplex virus (HSV) latency in primary neurons that mimics many aspects of HSV latency in animal models and the human disease (1–3). Using this model, we demonstrated that HSV-1 and HSV-2 establish latent infections in vitro in the same neuronal cell types th ...

Assays in use for the analysis of herpes simplex virus (HSV) gene expression during the establishment and maintenance phases of infection in the nervous system include: 1 The use of reporter genes, for example, the lacZ gene from Escher

Protective immunity against a eajority of viral infections is mediated by a combination of both humoral and cell-mediated immune responses. However, in the case of herpesvirus infections, where viral spread is largely cell-to-cell, cell-mediated immune mechanisms (which facilit ...

The polymerase chain reaction (PCR) technique is a sensitive method for detection of nucleic acids that can be used to detect herpes simplex virus (HSV)-DNA and RNA in tissue samples with greater sensitivity than hybridization with specific probes (1,2). In its most basic form, PCR involves mul ...

Herpes simplex virus (HSV) is a human pathogen that causes diseases ranging in medical importance from herpes labialis, through genital herpes and herpes keratitis, to herpes encephalitis-a life-threatening disease. HSV types 1 and 2 have the ability to enter a latent phase during in vivo inf ...

A number of aspects of the natural biology of herpes simplex virus (HSV) make it an attractive candidate for a vector to express foreign genes within the nervous system. Some of the advantages of an HSV vector are 1 Establishment of a l

This chapter deals with assays commonly used to follow herpes simplex virus type 1 (HSV-1) entry into and spread between cells in tissue culture These are complex processes, known to involve several of the 20 or more HSV-encoded membrane proteins (see refs. 1 and 2 for recent reviews). HSV entry is media ...

This chapter deals with (1) the preparation of herpes simplex virus (HSV) virion DNA of a quality and purity suitable to be used for the generation of infectious virus, and (2) its use in the preparation of infectious virus. An important development in the understanding of virus genetics and gene prod ...

The study of genome structure and gene function is pivotal in understanding the mechanisms of replication, pathogenesis, and virulence of herpesviruses. In this respect, mutagenesis and sequence analysis of genes encoded by the virus are of great importance. However, the herpesvirus g ...

Complementary cell lines have become an accepted tool for the functional analysis of viral genes (1,2) and proteins, as well as an essential component in strategies for the construction of mutant viruses. More recent applications include the propagation of replication-defective vir ...

Lytic infection by herpes simplex virus (HSV) efficiently inhibits the synthesis of most cellular proteins while a large number of viral proteins is produced, including a host shut off protein (1–3). The inhibitory effect of the protein obviously needs a certain period of time to be fully effici ...

The isolation of an individual polypeptide from a heterogeneous mix is an essential process in characterizing a protein of interest. In purified form a protein can be used to generate specific polyclonal and monoclonal antibodies for in vivo studies, in vitro the enzymic properties or inter ...

Herpes simplex virus (HSV) remains a major human pathogen worldwide (25 causing cold sores, eye and genital infections, blindness, encephalitis, and neonatal infections. Most adults have antibodies against the oral form of the virus HSV-1 (9), and a significant number are infected with the g ...

Proteomics has been widely applied to develop two-dimensional polyacrylamide gel electrophoresis maps and databases, evaluate gene expression profiles under different environmental conditions, assess global changes associated with specific mutations, and define dr ...

Two-dimensional electrophoresis results in an adequate resolution of the proteome of microorganisms to allow the detection and identification of specific antigens after blotting on membranes and overlaying the protein pattern with patient’s sera. The complement of all identi ...