分子实验

Based on Krapp at al (1993) Plant Journal 3:817 with modifications. Comments or Questions? Send to me at: chunming.liu@wur.nl ------------------------------------------------------------------------ ...

This method is derived from a procedure developed by Toby Bradshaw and the Poplar Molecular Genetics Cooperative. We have tested the procedure with a variety of Populus species as well as tobacco and ...

Procedure Preheat Extraction Buffer at 60°C. Weigh 100 mg of fresh leaf tissue and grind it to powder in Liquid Nitrogen in a chilled mortar and pestle. Add 0.5 ml of TE buffer to the tube. Ce ...

Solutions: Extraction buffer: 200 mM Tris-HCl pH 7.5 250 mM NaCl 25 mM EDTA 0.5% SDS Arial He ...

A protocol / method / schedule /procedure for extraction / isolation of both DNA and RNA from the same material typically plant leaf / leaves (See also DNA Isolation protocol) 1) Take one medium siz ...

CTAB TECHNIQUE / Method / Schedule / Protocol FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES (see also DNA RNA double isolation procedure if both DNA and RNA are needed) Reagent ...

1.collect piece of tissue (e.g. pieces of a leaf or flower) in Eppendorf tube containing 40 ml 0.25 N NaOH 2.put in boiling H2O for 30 sec (optimum may need to be determined for each type of tissue f ...

一、实验目的 观察玉米籽粒性状间的连锁遗传现象；理解连锁和交换的原理；掌握测定基因间交换值和基因定位的方法。 二、实验原理 位于同一染色体上的两非等位基因(如AB或ab)，总是有联系在一起分配到同一配子中去的倾向。若两非等位基因完全连锁，杂合体(AB//ab)只产生2种亲本型配子(AB和ab)，F2代出现与杂交亲本相同的2种表型。若两非等位基因不完全连锁，杂合体(AB//ab)仍产生4 ...

一、实验目的 学会利用所学知识，进行自主实验设计；了解染色体畸变的各种类型及原理，掌握利用微核检测技术监测环境污染的一般方法。 二、实验原理 微核（micronucleus 简称MCN），也叫卫星核，是真核类生物细胞中的一种异常结构，是染色体畸变在间期细胞中的一种表现形式。微核往往是各种理化因子，如辐射、化学药剂对分裂细胞作用而产生的。在细胞间期，微核呈圆形或椭圆形，游离于主核之外，大小应在主 ...

1.protocol (1)collect piece of tissue (e.g. pieces of a leaf or flower) in Eppendorf tube containing 40 ml 0.25 N NaOH (2)put in boiling H2Ofor 30 sec (optimum may need to be determined for each type ...

PCR反应的最大特点是具有较大扩增能力与极高的灵敏性，但令人头痛的问题是易污染，极其微量的污染即可造成假阳性的产生. 一、污染原因 1、标本间交叉污染:标本污染主要有收集标本的容器被污染，或标本放置时，由于密封不严溢于容器外，或容器外粘有标本而造成相互间交叉污染;标本核酸模板在提取过程中，由于吸样枪污染导致标本间污染;有些微生物标本尤其是病毒可随气溶胶或形成气溶胶而扩散，导致彼此间的污染. 2、P ...