Genomic DNA Extraction for Mapping

Solutions:

Extraction buffer: 200 mM Tris-HCl pH 7.5, 250 mM NaCl, 25 mM EDTA, 0.5% SDS

Extraction Buffer   1 M Tris-HCl pH 7.5 10.00 ml 5 M NaCl 2.50 ml 0.5 M EDTA 2.50 ml 20% SDS 1.25 ml H 2 O 33.75 ml Total 50.00 ml

Remarks:

This protocol is quick, reliable and the DNA obtained can be used for PCR-based mapping using SSLP and CAPS markers. It is based on the protocol by Edwards et.al., 1991, NAR 19: 1349. It was adapted for large-scale applications by the Somerville lab.

Materials:

Reagent/Tool	Supplier	Cat.-#