化学生物学实验技术

Immobilized cyclodextrin derivatives are used as chiral selectors in various modes of capillary electrochromatography (CEC). The present chapter describes three techniques in detail utilizing CDs in CEC: (1) open-tubular capillary electrochromatography (o-CEC), (2) pack ...

As the most popular chiral selectors, β-cyclodextrin (β-CD) and its derivatives have been widely used in enantiomer separations. Among functionalized β-CDs, sulfated β-CDs are recognized as the most effective chiral additives in capillary electrophoresis (CE) separations. Here ...

A generalized preparation protocol for open tubular (OT) molecular imprinted polymer (MIP) silica capillary columns for chiral separations in capillary electrochromatography (CEC) is described. For �different chiral molecules the same general composition of the MIP reacti ...

An up-to-date overview of thin-layer chromatography (TLC) techniques for chiral separations of various significant and/or recent examples of enantioresolutions is reported. Furthermore, examples for chiral separations obtained on achiral commercially available C18 TLC ...

Capillary electrochromatography (CEC) has been shown to be a promising technique for miniaturized enantioseparations. Here we present the preparation and application of positively charged cellulose derivative-based chiral stationary phases (CSPs) for the CEC separation ...

The chiral separation principle ligand-exchange is applicable for HPLC, capillary electrophoresis as well as for capillary electrochromatography. Among other chiral separation principles, ligand-exchange permits simple method optimization and can be used particula ...

Capillary electrophoresis-mass spectrometry (CE-MS) is a powerful analytical tool, especially in the case of chiral separations, due to the fact that it combines the high efficiency, short analysis time, and versatility of the CE with the sensitivity, selectivity, and the capacity for the ...

Chiral ligand-exchange capillary electrophoresis is a relatively cheap and flexible format of capillary electrophoresis. It separates chiral analytes with a metal complex chiral selector that is composed of a metal ion and either homogeneous or heterogeneous chiral ligands (in r ...

In this chapter, an overview of experimental designs to develop chiral capillary electrophoresis (CE) and capillary electrochromatographic (CEC) methods is presented. Method development is generally divided into technique selection, method optimization, and method vali ...

Capillary electrophoresis (CE) methods using proteins as the chiral selectors have been developed for the separation of enantiomeric mixtures. For chiral separations in protein-based CE, two methods were utilized. One is affinity capillary electrochromatography (ACEC), and t ...

Chiral separation by means of cyclodextrins has a long-standing tradition in capillary electrophoresis techniques. Here we present a chiral method utilizing the recently introduced microemulsion electrokinetic chromatography. The microemulsion consisting of 1.0% SDS, 4 ...

The separation of enantiomers is one of the important fields of modern analytical chemistry, especially for agrochemical and pharmaceutical products because the stereochemistry has a significant influence on the biological activities of compounds. Cyclodextrin-modifi ...

The use of amino acid-based polymeric surfactants (a.k.a. molecular micelles) in chiral micellar electrokinetic chromatography (CMEKC) has been shown to be a successful separation mode for capillary electrophoresis (CE). In this mode, chiral compounds can be enantioseparated wi ...

Macrocyclic antibiotics, especially vancomycin, represent a class of versatile chiral selectors for enantioseparations by capillary electrophoresis (CE). In this chapter, we describe the protocol for performing CE enantioseparations with vancomycin as the chiral selec ...

Chiral recognition phenomena play an important role in nature as well as analytical separation sciences. In separation sciences such as chromatography and capillary electrophoresis, enantiospecific interactions between the enantiomers of an analyte and the chiral selector ...

The enantioseparation of acidic and basic compounds can be successfully achieved in nonaqueous capillary electrophoresis using single-isomer charged β-cyclodextrin (β-CD) derivatives of opposite charge to that of the analytes. This chapter describes how to separate the enan ...

The enantioseparation of acidic and neutral compounds can be successfully achieved in capillary electrophoresis (CE) using dual cyclodextrin (CD) systems. This chapter describes how to separate the enantiomers of acidic or neutral substances using dual CD systems made up of a negati ...

Due to their commercial availability, cyclodextrins are the most frequently used chiral selectors in capillary electrophoresis as documented by the numerous publications in the field. A variety of migration modes can be realized depending on the characteristics of the cyclodextr ...

Generally, in using chromatography as an industrial-scale production process, batch separation is not adequate from the standpoint of productivity and mobile phase consumption; thus, a continuous separation is preferred. The simulated moving bed (SMB) method is one of the best popul ...

Supercritical fluid chromatography is an environmentally benign separation technology due to the use of carbon dioxide modified with organic solvents as the mobile phase. It has found widespread use in the separation of chiral compounds based on the relative ease of method development ...