常用设备/其他

Quantitation of DNAFluorescent quantitation Wear gloves and goggles protecting from EtBr and UV light With an EDP (dilute mode) pick up 9 µl TE and 1 µl vortexed DNA sample. Spot this on a place of sc ...

Bradford Assay is a rapid and accurate method commonly used to determine the total protein concentration of a sample. The assay is based on the observation that the absorbance maximum for an acidic so ...

Bradford protein assayConsiderations for useThe Bradford assay is very fast and uses about the same amount of protein as the Lowry assay. It is fairly accurate and samples that are out of range can be ...

Bradford Protein Concentration Assayversion 01/07/2001Abbreviations:mcg = micrograms mcL = microliters BSA = bovine serum albumin O.D. = optical density dI = deionized BackgroundThe Bradford protein a ...

Bradford � Protein DeterminationIntroduction A rapid and accurate method for the estimation of protein concentration. The technique is simpler faster than the Lowry method and is subject to less inter ...

Protein AssaysBelow is a list of assays for the determination of protein concentration in a solution. This list includes the sensitivity range volume/amount of sample needed subjective comments on acc ...

Biorad Protein Assay: Bradford Standards: 1 mg/ml BSA stock- dilute 1:10 to get 0.1 mg/ml BSAAdd To get H-2O20 µl 2 µg/ml 780 µl40 µl 4 µg/ml 760 µl60 µl 6 µg/ml 740 µl80 µl 8 µg/ml 720 µl100 µl 10 µg ...

BIURET PROTEIN ASSAYMATERIALS Biuret Reagent Bovine serum albumin (BSA) Spectrophotometer and tubes PROCEDURE Prepare standard dilutions of BSA containing 1 2.5 5.0 7.5 and 10 mg/ml protein. Prepare s ...

COMPARISION OF DIFFERENT PROTEIN DETERMINATION METHODSCompanyMethodDetection RangeApplications -CompatibilityAssay protocolPrecautions-InterferencesAbsorbance 280nm0.1-1 OD280nm/mlAbsorbance of aroma ...

1.1. Can the NCIS Kit be used on adults?The NCIS kit is specifically packaged for five separate tissue dissociations each containing up to twelve neonatal rat hearts. The NCIS protocol is available on ...

Cell Isolation Techniques Methods and MaterialsWorking With EnzymesAll of the enzymes Worthington offers for tissue dissociation applications are available as lyophilized powders for convenience vers ...

Cell Isolation Techniques Methods and MaterialsWorking With EnzymesAll of the enzymes Worthington offers for tissue dissociation applications are available as lyophilized powders for convenience vers ...

Phosphoamino acid analysis of non-TCA precipitable proteins1. Cut out gel slice incubate 60 min with 1 ml 5.7 M HCl @ 110oC in 13 x 100 mm screw cap tubes. 2. Add 4 ml water centrifuge @ 2000 rpm in b ...

Phosphoamino acid analysis of non-TCA precipitable proteins1. Cut out gel slice incubate 60 min with 1 ml 5.7 M HCl @ 110oC in 13 x 100 mm screw cap tubes. 2. Add 4 ml water centrifuge @ 2000 rpm in b ...

Lowry � Protein Determination(From Protein Protocols on CD-ROM Humana Press 1998 - Section 1-2 The Lowry Method for Protein Quantitation Jakob H. Waterborg and Harry R. Matthews) (Lowry O. H. Rosebrou ...

Protein concentration of Laemmli gel samplesTo 10 µl boiled lysate (in Laemmli sample buffer) add 40µl water + 50µl 50% TCA. Ppt. 10 min. on ice. Spin and wash with 10% TCA. Resuspend pellet in 100 µl ...

Protein Assay (Spectrophotometer)Use BSA (bovine serum albumin) 1mg/ml stock solution (1ml Eppendorf tubes) for standard curve. Place 0 2 5 10 15 20µg (=µl) BSA into plastic cuvette. Use 2-5µl of samp ...

Purpose...Concentration of proteins in a crude preparations (such as culture sup) can be estimated semi-quantitatively by using "Dot Blot" method if you have both purified protein and specific antibod ...

Purpose...Concentration of proteins in a crude preparations (such as culture sup) can be estimated semi-quantitatively by using "Dot Blot" method if you have both purified protein and specific antibod ...

UV Absorbance (280 nm) � Protein Determination Simple and quick method to accurately quantitate total protein in purified material or approximately quantitate total protein in crude lysates or partia ...