常用设备/其他

Banker Culture Hippocampal Dissection 1) Begin by placing two large (100mm) and three small (60mm) petri dishes in an ice bath. Fill with cold HBSS medium (calcium and magnesium free). Place a large Falcon tube (50ml) containing fresh HBSS in bath.2) Euthanize the pregnant rat with halothane by plac ...

Adenovirus Cre Intranasal Infection Information & Protocol腺病毒鼻内感染小鼠 Jacks Lab, Center for cancer research,MIT http://web.mit.edu/ccr/labs/jacks/protocols/AdenovirusCre.htmlREAGENTS: MEM (Sigma catalog #M-0268) 2 M CaCl2 *Adenovirus - University of Iowa Notundefined- The U ...

Principles of SurgeryWelcome to the Principles of Surgery Home Page . The goal of this project is to aid students in mastering the skills and information presented in the course 'Principles of Surgery'. the principles of surgery home page is below:http://cal.vet.upenn.edu/surgery/ 上一篇：急性 ...

Procedures Common to the First Five Behavioral TestsMarking the miceOn Monday morning in the colony room, tail mark the 32 mice to be tested in the AM. Use a Sharpie pen (waterproof black or red) to make 1 or 2 tail bands near the base of the tail. Start at cage 1 and proceed in order to cage 16. Capture one mouse, then consult a l ...

Features of the Husbandry to be Equated Across LabsFive periods may differ in the requirements to some extent. Features mentioned only for the first period are common to all periods.After arrival from supplier but before mating; housed with same sexMated pairs before and after delivery of lit ...

Quick and simple mouse forebrain commissure anatomy1. Make solutions ahead of time2. Euthanasia - use either CO2 or pentobarbital sodium; consult a veterinarian3. Rapidly remove the brain from the skull.4. Fix the brain in 10% formalin or 4% paraformaldehyde for at least 24 hours.5. Trim and weigh ...

Protocol for Rotarod Coordination testingApparatus 2 SmartRods from Accuscan. Each box has a plastic bedding tray added that is about 2 cm above the photocell beams so that the mouse can never break the photocell beams. The tray should be filled with about 1cm of the standard 1/4" bed-o-cob bedding. The ...

Locomotor Activity Testing ProtocolApparatus and Room Setup1. Activity monitors are enclosed in a sound attenuating chamber with a ventilation fan on. Fan provides some masking noise. Fan is a common 3" or 4" muffin fan (110 VAC). We can try to define the actual sound level, but matching may not be easy sin ...

Protocol for Elevated Plus MazeBasic FeaturesThe test will be a five min test on the maze immediately following transport from the holding area in the home cage.General Apparatus Setup and Room Configuration1. Mazes are to be placed in an area where as much symmetry as possible is achieved from the r ...

Water Maze Escape Learning TaskPhysicalTank (provided by DW lab)Material polypropyleneColour whiteDiameter 70 cm insideHeight 20 cmRim L shaped, 3x3 cmWater (local tap water)Depth 15 cm; Volume =15 cm x (35 cm)2 = 57,727 cc = 57.7 LSurface to rim 5 cmTemperature 25 ± 1 COpacity For visible platfo ...

Cocaine-stimulated Locomotor Activity Testing ProtocolBackground DiscussionThis test should have the same length as the Monday activity test (15 min). Cocaine will be administered immediately prior to placement in the activity chamber.Otherwise the protocol here is the same w ...

Dissociated Cultures of Cerebellar Neurons分离培养大鼠或小鼠小脑神经元Protocol Dissection can do on counter (i.e., in non-sterile conditions)use P8 rat pups, or P5 mouse pupskeep on ice whenever possiblefor each step, do all pups, before moving to next stepfill 2 tubes with approx. 30ml HHGN (one for di ...

Investigating the behavioral displays of male Siamese fighting fish, Betta splendensIntroductionSiamese fighting fish (Suborder Anabantoidei) have long been valued by aquarists for their brilliant colors, ornate fin shapes, and stylized behavioral displays. Original ...

Alcohol ConsumptionOverall: Two days of water, followed by two, 2-day choices of 6% ethanol vs. water, with a position change between days 1-2 and 3-4.After the cocaine test of Friday, mice are placed by pairs into a new home cage (i.e., bedding change) with a water bottle that has a sipper tube (something that Po ...

Beta-Gal staining in primary neuronsFix cells w/ 4 ml fix soln/10cm or 60 cm dish, 37 C, 5 min Fix soln.: for 100 ml (use w/i 1 wk) 5.4 ml 37 % formaldehyde (Final 2%) 800 ul 25% Glutaraldehyde (fin. 0.2%) 94.5 ml PBSWash cells 1x w/ PBS prewarmed to 37 C Stain w/ 2 ml staining soln./60 mm plate for 30 min to o/n. Blue cells may start to appear in 30 min. St ...

Co-staining with Thioflavin S & Carboxyfluorescein1.) Prepare 0.01% thioflavin S (T1892 , Sigma) solution in F12+ 2.) Filter the staining solution with a 0.22 µm Millipore filter.3.) Incubate the staining solution and F12+ wash for approximately 30 minutes to adjust solution temperature to 37 ...

脑皮质细胞分离培养 Culture of Dissociated Cortical Neurons1. Use 7 to 8 day chicken embryos2. Dissect forebrain in Ham's F12 media, peel off all meninges and then separate cortex from basal ganglia3. Dissociate neurons by incubating with 0.5% trypsin for 20 minutes at 37°C4. Wash tissue with fetal calf ser ...

现代神经科学研究技术 Modern Techniques in Neuroscience Research现代神经科学研究技术Springer-Verlag GmbH & Co.KGPDF格式压缩文件，20M，点击此处下载Chapter 1Cytological Staining MethodsChapter 2Application of Differential Display and Serial Analysisof Gene Expression in the Nervous Syst ...

Pichia酵母密码子表氨基酸密码频率氨基酸密码频率LeuTTA15.6HisCAT11.9TTG31.2CAC9.1CTT15.7GlnCAA25.3CTC7.5CAG16.2CTA10.9AsnAAT24.6CTG15.1AAC26.1IleATT30.8LysAAA30.4ATC19.6AAG34.7ATA11.7AspGAT36.0MetATG18.1GAC26.2ValGTT27.1GluGAA39.0GTC ...

内切酶数据库 The Restriction Enzyme Database (REBASE)NEB公司内切酶数据库http://rebase.neb.com/rebase/rebase.html 上一篇：密码子数据库 Codon Usage Database 下一篇：P32放射自显影曝光时间 P32 Autoradiographic Exposure Times