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        Features of the Husbandry to be Equated Across Labs

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        682
         

        Features of the Husbandry to be Equated Across Labs

         

        Five periods may differ in the requirements to some extent. Features mentioned only for the first period are common to all periods.

        After arrival from supplier but before mating; housed with same sex

        Mated pairs before and after delivery of litter

        Weaned offspring before the start of behavioral testing

        Preparation for the formal behavioral testing - may be specific to this study

        During behavioral testing


         

        After arrival

         

        Food Purina 5001 (4.5% fat)

        Bedding Bed-O-Cob 1/4"

        Nesting material none

        Cage top Stainless steel bars (plus passive filter top in Portland)

        Animal handling Gloved hand

        Animal marking nil

        Animal weighing nil

        Mice/cage 4 or 5 maximum

        Lights on/off 0600/1800


         

        Mated pairs (items the same as after arrival are not repeated)

         

        Food Purina 5020 (9% fat)

        Nesting material 1 Nestlet

        Marking none

        Weighing at mating

        Mice/cage 1 female, 1 male

        Male present with litter? Yes; postpartum pregnancy allowed

        Inspection of new litter Count live and dead pups; remove dead, put live in nest

        Culling of newborns Remove only the obvious runts for euthanasia

        Cage changing with litter Do not disturb cage until litter is 4 days old; then clean cage, including fresh nestlet

        Weaned offspring

         

        Age at weaning Aim for 21 days (may be ± 1 day when weekend involved)

        Weighing Record body weight to nearest 0.5g; find means for each sex

        Food Purina 5001

        Mice/cage Same sex littermates, 4 or 5 maximum

        Marking nil


         

        About two weeks before the start of behavioral testing

        Mice/cage Cull to 2 by random lottery (keep some spares in reserve)

        Cage changing day Friday


         

        During behavioral testing

        Marking Sharpie pen (black or red) on tail; start Monday AM

        Weighing On Friday before the coke test

        Handling Hand with plastic surgical glove

        Cage changing day Friday (AFTER coke test trial for each pair)

        <center> <p>  </p> </center>
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