常用设备/其他

实验动物饲育与管理实验动物饲育与管理何新桥 金玫蕾等 编著科学出版社，1998年PDF格式，7.5M，点击此处下载内容简介：本书共分四篇，第一篇简要介绍生物学基础理论知识，如实验动物学，遗传学、微生物学、寄生虫学等；第二篇在论述实验动物技术应用方面着重传授鼠、兔、犬、猫、猕猴等动物的繁育方法；第三篇主要阐述实验动物疾病与控制；第四篇概括介绍现代化屏蔽系统实验动物的饲养技术。 上一篇：转基因鼠--Transgenic Mouse-Met ...

普鲁卡因小鼠腹腔注射LD50的测定广东药学院 谭毓治 【目的】通过实验学习测定药物LD50的方法、步骤及计算过程，观察受试药品一次给予动物后所产生的急性毒性反应和死亡情况。 【原理】药物给药剂量与动物死亡率间呈正态分布，以对数剂量为横坐标、死亡率为纵坐标作图，可得到一对称S型曲线，其两端较平坦，中间较陡，说明两端处剂量稍有变化时死亡率的改变不易表现出来，在50%死亡率处斜率最大，该处剂量稍有变动时，其死亡率变动最明显，即最灵 ...

基因敲除--Gene Knockout ProtocolsMethods in Molecular Biology VOLUME158Gene Knockout Protocols 基因敲除实验方法M. J. Tymms and I. KolaHumana Press Inc.PDF格式，共437页，4.5M，点击此处下载内容：1 Overview Gene Knockouts2 Isolation and Maintenance of Primate Embryonic Stem Cells3 Gene Targeti ...

生物样品的采集及实验动物的解剖和脏器系数测定中国药科大学 季晖一、意义在药物毒理学研究中，常常需要收集实验动物的血液、尿液或其它体液进行常规检查或生化分析，因此，正确采集实验动物的生物材料是药物毒理学最基本和最重要的操作技术。对实验动物进行大体解剖检查是药物毒理实验的常规观察项目，简便易行并能提供重要资料。测定动物死后器官湿重和含水量是常用的指标之一，可以从大体标本大概地估计内脏器官病变的程度，特别适用于某些可 ...

一、采血(一)剪尾采血1、剪尾采血：需血量较少时常用此法。先将动物固定，将鼠尾浸在45℃左右温水中几分钟或用酒精棉球涂擦鼠尾，使尾部血管充盈，剪去尾尖1-2mm(小鼠)或3―5mm(大鼠)，使血液顺血管壁自由流入试管或用血红蛋白吸管吸取。采血结束时，伤口消毒并压迫止血。小鼠每次可取血0．1ml左右，大鼠可取血0．3―0．5ml。2、内眦取血：操作者―手固定小鼠或大鼠，食指和拇指轻轻压迫颈部两侧，使眶后动静脉充血，另一只手持毛细采 ...

Bradfield Lab, McArdle Laboratory for Cancer Research, University of Wisconsin-Madison Medical School http://mcardle.oncology.wisc.edu/bradfield/Ahr-transgenic-genotyping.htmlAmplify a 600bp fragment using OL638 and OL639. Run 10 ul from each PCR reaction out on a 1% ag ...

实验动物的给药途径和方法及药量计算方法 一．经口给药法(一)灌胃法此法给药剂量准确，是借灌胃器将药物直接灌到动物胃内的・种常用给药法。1．鼠类：鼠类的灌胃器由特殊的灌胃针构成。左手固定鼠，右手持灌胃器，将灌胃针从鼠的右U角中，插入口中，沿咽后壁慢慢插入食道，使其前端到达膈肌位置，灌胃�插入时应无阻力，如有阻力或动物挣扎则应退针或将针拔出，以免损伤、穿破食道或误入气管。2．兔、犬等：灌胃一般要借助于开口器、灌胃管进行。先将动物 ...

Bradfield Lab, McArdle Laboratory for Cancer Research, University of Wisconsin-Madison Medical School http://mcardle.oncology.wisc.edu/bradfield/Cyp1a2-genotyping.htmlThis protocol uses Ampitaq Gold from Perkin-Elmer, which eliminates the need for a manual hot ...

Bradfield Lab, McArdle Laboratory for Cancer Research, University of Wisconsin-Madison Medical School http://mcardle.oncology.wisc.edu/bradfield/ARNT-transgenic-genotyping.htmlAmplify a 700bp fragment using OL637 and OL639. Run 10 ul from each reaction on a 1% agaro ...

Bradfield Lab, McArdle Laboratory for Cancer Research, University of Wisconsin-Madison Medical Schoolhttp://mcardle.oncology.wisc.edu/bradfield/ARNT-Knockout.htmlAmplify a 459bp fragment of neomycin using OL855 and OL856. The neomycin gene will only be present in t ...

Bradfield Lab, McArdle Laboratory for Cancer Research, University of Wisconsin-Madison Medical Schoolhttp://mcardle.oncology.wisc.edu/bradfield/Ahr-knockout.htmlThis protocol is published in Journal of Biological Chemistry (1993) 268:22203-22209. PCR a 218 ...

Materials8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C � do not go higher). Add a few drops of 2 N sodium hydroxide until the solution clears. Make fresh each day. Alternatively, 8% paraformaldehyde can be purchased from Elect ...

Mini-Chamber for regulating gaseous environment during culture 一个简单又省钱的方法来调节培养板的气体环境 A simple and inexpensive chamber for regulating gaseous environment of small culture plates, such as those used for culture of preimplantation embryos, can be constructed using disposa ...

Procedures obtained from Ann Croy (Guelph, Canada) as a modification of procedures detailed in Transplantation 1971 12:148-151 Takasugi, M. An improved fluorochromatic cytotoxic test. Note that the procedure can be used for staining of all cells and not just embryos or oocytes. Also, ethid ...

Bovine Rectal PalpationAnatomical Basis of Rectal Palpation Manipulation and examination of the reproductive tract of cattle via rectal palpation is possible because of several anatomical features of the cow:1) As in other species, the reproductive tract of the bovine is suspended f ...

The FAM caspase binding assay kits from ATCC Corporation can be used to determine amounts of active caspases in cells. The FAM-labeled caspase inhibitor can freely diffuse into the cell. Active caspase irreversibly binds the inhibitor. Upon washing the cells, the amount of fluorescence is pr ...

Differential staining of trophectoderm and inner cell mass in combination with TUNEL labeling of bovine embryos将滋养层和内细胞群分别染色，TUNEL检测牛胚胎凋亡Simple protocol for differential staining/TUNEL analysis of bovine embryosThe procedure for differential staining of troph ...

Making collagen solution from lyophilized rat tail collagen鼠尾制备胶原蛋白Chen-Ming Fan Lab,Carnegie Institute of Washington(lyophilized rat tail collagen from Roche)DME power from Life technologies (buy the one withoutNaHCO3, make a 10X solution but with the pH color indicator, an ...

Genotyping from mouse tailsChen-Ming Fan Lab,Carnegie Institute of Washington(Laura’s method)All pipets must be filter tippedBe very careful to prevent cross contamination of samplesChange gloves oftenDo not vortex the tubes1. Place tail tip in 500 uL of Transgenic lysis buffer (m ...

Embedding, Sectioning, and Mounting of Frozen TissuesChen-Ming Fan Lab,Carnegie Institute of Washington Embedding of Frozen Tissues.Let PFA fixed embryos sink to the bottom of a tube containing a 30% sucrose (inPBS) solution overnight. After they have sunk, remove all but 2-3 mL's of thesol ...