常用设备/其他

氨基酸的物理常数 中文名称英文名称（缩写）M熔点/℃①溶解度②等电点pKa（0℃） DL-丙氨酸DL-alanine(Ala)89.09295d16.66.00(1)2.35(2)9.69 L-丙氨酸L-anginine(Alg)89.09297d16.656.00 DL-精氨酸DL-arginine(Arg)174.20238d10.76(1)2.17(COOH)(2)9.04(NH2)(3)12.48(胍基) L-精氨酸L-arginine（Arg）174.2024 ...

DNA 摩尔换算 1ug1000bp DNA=1.52pmol=3.03pmol 末端 1ugpBR322DNA=0.36pmol 1pmol pBR322=2.8ug 1kb 双链 DNA( 钠盐 ) ＝ 6.6 10 5 dalton 1kb 单链 DNA( 钠盐 ) ＝ 3.3 10 5 dalton 1kb 单链 DNA( 钠盐 ) ＝ 3.4 10 5 dalton 脱氧核糖核苷的平均 Mr=324.5dalton蛋白质 /DNA 换算 1kb DNA =333 个氨基酸编码容量 =3.7 10 4 Mr蛋白 10000 Mr蛋白质=270 bpDNA 30000 Mr蛋白质=810 bpDNA 50000 Mr蛋白质=1.35 kp ...

实验室中常用酸碱的比重和浓度名称 分子式 分子量 比重 面分浓度 % （ W/W ） 当量浓度（粗略） N 配 1 升 1N 溶液所需毫升数 盐酸 HCl 36.47 1.19 37.2 12.0 84 1.18 35.4 11.8 1.10 20.0 6.0 硫酸 H2SO498.09 1.84 95.6 36.0 28 1.18 24.8 6.0 硝酸 HNO263.02 1.42 70.98 16.0 63 1.40 65.3 14.5 1.20 32.36 6.1 冰乙酸 CH3COOH 60.05 1.05 99.5 17.4 59 乙酸 CH3COOH 98.06 36 6.0 磷酸 H2PO435.05 1.71 85.0 15,30,45 ( 依反应而定 ) 67 ( 以 15N 计 ) 氨水 NH4OH0.90 15 67 0.904 27 ...

几种常用的酸碱指示剂指示剂名称 配制方法 颜色 变色 pH范围 中文 英文 0.1g溶于205mL下列溶剂 酸 碱 甲酚红（酸范围） Cresol red (acid range) 水，含 2.62mL 0.1mol/LNaOH 红 黄 0.2-1.8 间苯甲酚紫（酸范围） m-Cresol purple (acid range) 水，含 2.72mL 0.1mol/LNaOH 红 黄 1.0-2.6 麝香草酚蓝（酸范围） Thymol blue (acid range) 水，含 2.15mL 0.1mol/LNaOH 红 黄 1.2-2.8 金莲橙 OO 水 红 黄 1.3-3.0 甲基黄 90％乙醇 红 黄 2.9-4.0 溴酚蓝 水，含 1.49mL 0. ...

调整硫酸铵溶液饱和度计算表（25℃）

1．0．1％焦碳酸二乙酯(DEPC)处理水 在1 L蒸馏水中加入1 mL DEPC，在磁力搅拌器上搅拌过夜，分装后高压灭菌30 rIlin备用。 2．5 X甲醛凝胶电泳缓冲液 0．1 mol／L MOPS 40 mmol／L NaAC 5 mmol／L EDTA(pH 8．0) 3．10 x RNA加样缓冲液 50％甘油 1 mmol／L EDTA(pH 8．0) 0．25％二甲苯青 0．25％溴酚蓝 4．6×DNA加样缓冲液 40％(质量／体积分数)蔗糖 0．25％二甲苯青 0．25％溴酚蓝 5．5 X第一链反应缓冲液 250 mmol／L Tris―HCl(pH 8．3) 375 mmo ...

磷酸缓冲盐(Phosphate―buffered saline，PBS，pH 7．4)溶液的配制和各种pH值Tris-HCl缓冲液的配制 1．PBS(pH 7.4)的配制 NaCl 8.0 g KCl 0.2 g Na2HP04・12H20 2.9 g(或Na2HP04加1.15 g或Na2HP04・2H20加1.44 g) KH2P04 0.2 g 双蒸水 加至1 000 mL 以上各试剂依次溶解在800 mL双蒸水中，用15％NaHC03或1 moL／L HCl调pH值后，再加水定容至1000 mL 2．各种pH值(0.05 moL) ...

常用植物生长物质的理化性名称相对分子质量理化性质吲哚乙酸(1AA)175.19无色结晶，见光易分解，熔点164℃～165C．，微溶于水，易溶于乙醇、乙醚和丙酮。在碱性溶液中比较稳定萘乙酸(NAA)186．2无色针状结晶，熔点134．5r一135．5℃，易溶于乙醇、丙酮、乙醚等。微溶于热水，易潮解和见光易变色吲哚丁酸(1BA)203．2白色或微黄色结晶，稍有异臭，熔点为123℃～125℃，不溶于水，能溶于醇、醚、丙酮等有机溶剂6―苄基 ...

Reagents:Plasmids: can be found in my plasmid(1) box in the ?20oC freezer.985: FR-tk-luciferase2517: Renilla-tk-luciferase2145: GFPLipfectamine and lipofectamine plus reagents: in the upper shelf of the medium freezer in tissue culture room.DMEM-10D20F: 100ul for one wellProt ...

HOW TO PERFORM AN INTERRUPT OF ANONGOING AUTOCOUNT1). This program interrupts the ongoing autocount to allow the counting of asmall number of vials- only one rack of vials. If you have more than onerack to count, think creatively and I'm sure you'll figure out how to countthem all with the help of the interr ...

目镜测微尺量测倍率比照表线状倍率目镜一格单位(mm)一格单位(μm)4100.0250 mm25.0 μm10100.0100 mm10.0 μm20100.0050 mm5.0 μm40100.0025 mm2.5 μm100100.0010 mm1.0 μm格状倍率目镜一格单位(长x宽) (mm)一格单位(μm)4100.25 mm x 0.25 mm250 μm x 250 μm10100.1 mm x 0.1 mm100 μm x 100 μm20100.05 mm x 0.05 mm50 μm x 50 μm40100.025 mm x 0.025 mm25 μm x 25 μm1 ...

In this Chapter the actual procedure of making a gigaseal on a cellmembrane and establishing the desired configuration is discussed. Thepurpose of this is to indicate good practice. Practitioners might find thatthis Chapter helps to increase the success rate, facilitates trouble-sh ...

Single-channel Protocols and Data Analysis PDF格式文件（570kb）6 Single-channel Protocols and Data Analysis 1416.1 General Single-channel Practice and Analysis 1416.1.1 Practical notes 1416.1.2 Amplitude analysis 1436.1.3 Event detection 148vi CONTENTS6.1.4 Dwell time anal ...

Basic Theoretical Principles（PDF格式560kb，点击左侧图标下载查看）This chapter deals with the combination of cell anatomical features andphysical and chemical properties of the cell environment that contribute tothe physiology of ion transport across the cell membrane. Starting fr ...

Although patch clamp set-ups range from a simple rig to the mostelaborate patch clamp arrangements in which a large number of variablesare carefully controlled, there is a basic set of conditions that must be metin all cases for patch clamping to work. These conditions will be discussedin this Ch ...

startup• Turn on the scope (switch on base, toward back, on the right).• Turn on the mercury lamp for the scope (small white box to the left of the scope).• Turn on the green laser (far right) by pushing the red button, turning the key to the right, and turning up the power to about 10 o’clock on the dial.• Turn on the red laser (next to g ...

1. Cleaning coverslips (use gloves)Add 1 bottle of Chromerge, 1/5 at a time, into 2.5 L bottle of Sulfuric Acid. Invert the bottle to mix after each addition.Load coverslips into glass carriers in every other slot at a slant (see fig. 1).Pour enough chromic acid solution into tray to cover the tops of the covers ...

Make-up 300 ml of 2 parts Nitric Acid - 1 part HCl in a glass beaker in the hood. Solution will turn orange-red. Place #1.5 coverslips into solution a few at a time. Let sit for about 2 hrs. with occasional swirl. Decant acid into waste bottle carefully. Wash extensively in running water until pH of water is back up to 5.5 -6.0. S ...

The light microscope, so called because it employs visible light to detect small objects, is probably the most well-known and well-used research tool in biology. Yet, many students and teachers are unaware of the full range of features that are available in light microscopes. Since the cost of an ins ...

The use of radioactive tracers in cell research is an effective and safe means of monitoring molecular interactions. There is simply no other technique which allows the precision and specificity of radioactive tracers. Radiation is to be taken seriously. At a minimum, its misuse can lead to incr ...