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        The Practice of Patch Clamping--膜片钳操作

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        1373
         
        In this Chapter the actual procedure of making a gigaseal on a cell
        membrane and establishing the desired configuration is discussed. The
        purpose of this is to indicate good practice. Practitioners might find that
        this Chapter helps to increase the success rate, facilitates trouble-shooting
        and reduces having to ‘reinvent the wheel’. Novices hopefully will be saved
        much time. The method used in this Chapter is to provide a step-by-step
        account of how to establish a patch clamp configuration, using the
        background information outlined in the previous Chapters. Electrophysiology
        is one of the disciplines within biology that allow many instant
        quality controls; these will be indicated where appropriate.
         
        4 The Practice of Patch Clamping 95
        4.1 Preparing the Experiment and Making a Seal 95
        4.1.1 Setting up 95
        4.1.2 Bringing the pipette near the preparation 98
        4.1.3 Making the seal 101
        4.2 Whole-cell Modes 104
        4.2.1 Conventional whole-cell recording 104
        4.2.2 Perforated patch recording 108
        4.3 Single-channel Modes 110
        4.3.1 General notes 110
        4.3.2 Cell-attached patch 112
        4.3.3 Excised patches 113
        <center> <p>  </p> </center>
        上一篇:How to interrupt scintillation?   下一篇:膜片钳实验所需器材物品
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