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Microwave Tissue ProcessingFrom Donna Willis Harris Methodist Hospital Ft. Worth TexasPURPOSE: To provide rapid tissue processing that will decrease turn-around-time.PRINCIPLE: Microwave exposure is u ...

Microwave Processing Schedules for BiopsiesProcedure IIIUsed by Denise Hardy ARUP Laboratories Salt Lake City UT. Pre-fix room temperature 1 hour. Microwave in reagent alcohol 67°C 5 minutes. Microwa ...

Incubate sections in the incubating medium at 37°C for 5 - 12 hours. Long incubation periods are needed to get significantly visible reaction product. Wash in distilled water for 2 minutes. Countersta ...

Using the Components The basis solution Technovit 9100 NEW can be used in stabilized or destabilized form. The use of destabilized Technovit 9100 NEW basis solution guarantees results for all immunohi ...

Alkaline Phosphatase for Glycol Methacrylate SectionsProcedure:Incubate sections in the incubating medium at room temperature for 1 - 3 hours. Two hours is sufficient in most cases. Wash in distilled ...

Glycol Methacrylate Embedding for ImmunohistochemistryTechnovit Glycol Methacrylate: Technical Information and Help From Heraeus Kulzer.Embedding in plastic provides many advantages to the histotechno ...

Glycol Methacylate Embedding for Materials SamplesTechnovit Glycol Methacrylate: Technical Information and Help From Heraeus Kulzer.Embedding in a plastic often supplies the support necessary to succe ...

Staining Protocols for Lymphoid Tissue:Technovit Glycol Methacrylate: Technical Information and Help From Heraeus Kulzer.Normal immunostaining procedures for routine markers on sections obtained from ...

COMBINED ALCIAN BLUE - P.A.S SECTIONS4micron paraffin wax sectionsSOLUTIONS1. Alcian Blue pH 2.5Alcian Blue 1g3% Acetic Acid 100ml2. 1% Periodic Acid 3. Schiffs ReagentMETHODTake sections to waterPlac ...

Periodic Acid Schiff Stain for Glycol Methacrylate Sections Technovit Glycol Methacrylate: Technical Information and Help From Heraeus Kulzer.Procedure: Oxidize sections in 0.4% periodic acid for 30 m ...

Sectioning and Mounting ProtocolTechnovit Glycol Methacrylate: Technical Information and Help From Heraeus Kulzer. SectioningSectioning is best done with a rotary or sledge microtome such as the JB-4 ...

for the technique of embedding specimens in plasticDay 1Step 1: Read and understand the Material Safety Data Sheets associated with each of the chemicals used in this procedure. Use of rubber gloves l ...

1.Deparaffinize slides a (after drying thoroughly overnight at RT) in 2 changes of xylene (or xylene substitute) for 10 mins each. 2.Transfer slides to 100% alcohol 2 changes for at least 2 m ...

Preparation and Staining of Paraffin Sectionsfor use with Pharmingen reagents I. Fixation and Processing of Tissue for Paraffin SectionsA. Fixation of Tissues in 10% Neutral Buffered FormalinSacrifice ...

Preparation and Staining of Frozen Tissue Sectionsfor use with Pharmingen reagents I. Preparation of Frozen Sections for SectioningMaterials neeed:2-methylbutane (isopentane) Liquid Nitrogen Dry ice P ...

SECTION ADHESIVES It is advisable to use an adhesive to promote tissue attachment to the glass slides used in histological preparation. This is routinely achieved by the application of a smear of glyc ...

Material MEMPfa: 0.1M MOPS -- pH 7.4 2mM EGTA 1mM MgSO4 (typically this is made up as 10X MSalts. 4% paraformaldehyde (diluted from a frozen stock of 20%) (store MEMPfa at 4C and use within 1 day). ...

CryosectioningWhile the timing of the various steps in this protocol are probably not critical I tend to prefer to process the tissue all at once to ensure that RNA and/or proteins do not get degrade ...

All tissue types except muscle:Tissue should preferably be snap-frozen by quenching in liquid nitrogen. Quenching is accomplished by putting a small amount of OCT compound onto a cork disc placing the ...

All tissue types except muscle:Tissue should preferably be snap-frozen by quenching in liquid nitrogen. Quenching is accomplished by putting a small amount of OCT compound onto a cork disc placing the ...