• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Glycol Methacylate Embedding for Materials Samples

        互联网

        750

         

        Glycol Methacylate Embedding for Materials Samples

        Technovit Glycol Methacrylate: Technical Information and Help From Heraeus Kulzer.

        Embedding in a plastic often supplies the support necessary to successfully section various materials samples, especially porousand inhomogeous samples. GMA has been used to embed and section polymer resins, multilayer foils and films, paper, textiles and coatings in addition to a wide variety of biological material. The ability of this low viscosity resin to infiltrate effectively can make sectioning non-biological samples fast and economical.

        Preparation of this type of sample is slightly different than preparing biological samples.

        Procedure:

        A fixation step is, of course, not necessary for materials samples.

        Dehydration for GMA embedments does not have to be complete because of GMA's miscibility in water. Only if the sample is saturated with water should a schedule of increasing alcohol concentration be used. This will displace most of the water with a solvent that can be replaced with plastic. Use schedule as follows:

        Infiltration displaces the dehydration solvent with monomer prior to beginning the polymerization reaction. If you have a porous sample, infiltration may be needed. Nonporous samples can skip this step. Make up your infiltrating solution according to the directions from your kit. For reusable Teflon® molds for more effective heat removal are available from DDK. While complete elimination of air is not needed, sealing the molds with paraffin or plastic wrap is often done. Leave the samples at room temperature for 1 hour then at 37° C for one hour to complete polymerization.

        If your embedments cannot be mounted directly to the microtome, an adapter must be attached. Place the suitable Technovit 3040 is an inexpensive media prepared by mixing 3 parts of the dry component to 1 part of the liquid component. Pour it into the adapter and polymerization will be complete within 10 minutes. The adapters will be firmly attached through a copolymerization of the Technovit 3040 with the GMA.

        You are now ready to begin sectioning with glass.

          • 70% ethanol for 10 minutes.
          • 96% ethanol for 10 minutes.
          • 96% ethanol for 10 minutes.
          • absolute ethanol for 10 minutes.
          • 70% ethanol for 10 minutes.
          • 96% ethanol for 10 minutes.
          • 96% ethanol for 10 minutes.
          • absolute ethanol for 10 minutes.
      •  

        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序