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        for the technique of embedding specimens in plastic

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        for the technique of embedding specimens in plastic

        Day 1

        Use of rubber gloves, lab coat, and safety glasses is mandatory.


        for the technique of embedding specimens in plastic Step 2 : On working mat, place the following: metal disposal dish; label to be used for specimen; specimen in 100% glycerin; paper towels; tweezers to handle specimen; paper tent (for protecting solution from dust contamination); nail to be used as pointed probe, bottle of Bio-Plastic.

        for the technique of embedding specimens in plastic Step 2a : Remove specimen from 100% glycerin and place on paper towels. Mop up as much glycerin as you are able to from the specimen.

        for the technique of embedding specimens in plastic Step 2b : Pour approximately 100ml of Bio-Plastic from the bottle to the metal disposal dish. Gently place the specimen and the label into this uncatalyzed plastic solution. Allow to remain in solution overnight. This prepares the specimen and prevents contamination by glycerin. Place the paper tent over the specimen and container to prevent contamination.

        for the technique of embedding specimens in plastic Step 3 : On working mat, place the following: mold, beaker containing water, measuring cup or ruler/tape measure for measuring the cubic centimetre(s) of the mold, pen and paper.

        for the technique of embedding specimens in plastic Step 3a : Select the mold you will use for the specimen. Calculate the volume in cubic centimetres (cc) or measure the necessary millilitres (ml) required to fill the mold. [Recall: 1cc = 1ml.]

        for the technique of embedding specimens in plastic Step 4 : On working mat, place the following: mold, mold releasing agent, paint brush, metal disposal dish; paper tent; straw or other object (if you wish to have a hole running through the plastic block); stir stick (nail); dropper (for catalyst); bottle of catalyst; bottle of Bio-Plastic; knowledge of the proper amount of Bio-Plastic and catalyst to use. See table below:

        Table: Amount of chemicals required for preparing the liquid plastic.

         

         

         Type of Container

         Volume of Container

         Chemicals required (bioplastic + catalyst)

         Amount of chemical required for base layer

         Amount of chemical required for covering layer

         Pyrex glass dish  20cc or 20 ml

         Bio Plastic

        Catalyst

         10 ml

        8 drops

         10 ml

        8 drops

         Pyrex glass dish, custard type  60cc or 60 ml

         Bio Plastic

        Catalyst

         30 ml

        24 drops

         30 ml

        24 drops

         Metal cake tin  60cc or 60 ml

         Bio Plastic

        Catalyst

         30 ml

        24 drops

         30 ml

        24 drops


         

        Day 2

        Day 3

         


        stained & cleared fish

         

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