实验动物基本参数

Objective:To test the effects of UV radiation at 254nm on Sea Urchin embryo development after radiation at the 2-cell stage.Background:Sea Urchins exhibit radial holoblastic cleavage. The first and se ...

Objective: to test the effects of inhibiting the fast and slow blocks to polyspermy on sea urchin development. BackgroundSea urchin eggs release components of the egg jelly that attract sperm and indu ...

The Effects of NiCl2on Spicule FormationJessica Ann Billet Franklin and Marshall Class of 2000Background and ObjectiveSea urchins exhibit radial holoblastic cleavage eventually forming a blastula. Sho ...

ObjectiveTo observe the role sulfate plays in sea urchin gastrulation and to replicate the findings of Karp and Solursh that sea urchin embryos fail to gastrulate without sulfate. To test whether endo ...

ObjectiveThe purpose of this experiment is to determine the effects of calcium-free seawater on the development of sea urchin embryos. Embryos were placed in calcium-free sea after fertilization and a ...

Objective: to separate the Blastomere at different points in development and observe the growth of the resulting embryos. Procedure: We extractrf the gametes from the sea urchins fertilized the sea ur ...

Objective: This experiment aims to discover if temperature has an effect on the rates of sea urchin embryonic development. By altering the external environment the experiment will determine if tempera ...

Zebrafish will mate and deposit fertilized eggs on the bottom of the tank at 'dawn'. They can be accustomed to lay at any convenient time by keeping the room dark and lighting the tank with a timer. T ...

In order to repeat a published experiment or have someone else repeat yours it is important to use the same materials. For developmental studies that means knowing the precise point in development tha ...

AbstractPrevious studies by Stachel and colleagues indicate that lithium chloride induction of post-midblastular Brachydanio rerio embryos results in deficiencies in normal anterior-posterior developm ...

Objective The objective of the experiment is to determine the effects of ethanol exposure on the embryonic development of zebrafish through observation of physical defomities.IntroductionEmbryonic dev ...

IntroductionZebrafish have transparentembryos that develop outside the mother. They develop rapidly so that at 24 hours after fertilization the embryo has formed most of its tissue and organ primordia ...

Background:Zebrafishor the teleost fish Danio reriois a rapidly developing organism that is a popular species for studying vertebrate development. Cleavage in the Zebrafish only occurs in the blastodi ...

Gastrulation has been celled "the most important time of your life" (Lewis Wolpert); without it you would be flat like a pancake! Vertebrate gastrulation is the process by which the simple structure o ...

IntroductionIn early stages of development in amphibian embryos very few cells' fates are rigidly determined. However previous experiments show that cells in one area of the developing embryo are dete ...

EMBRYO CELL AND TISSUE CULTURE TECHNIQUESRearing solution: 10% Modified Steinberg's Solution. Operating solution:Full strength HBSt solution. Microsurgery Preparation 1)Place two embryos in the 60 mm ...

Objective:The overall objective of this laboratory experiment is to examine specification in the axolotl through transplantation of the gill organ field.Introduction:The axolotl (Ambystoma mexicanum) ...

From the various microsurgical procedures of Spemann and others several developmental principles have emerged about amphibian embryos. One of the most important discoveries was the understanding that ...

ObjectiveIn this experiment a region of the neural crest will be transplanted from one stage-fourteen embryo to another (Figure 1). To facilitate the visualization of the grafts and observe the cell f ...

IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative development at some point during their growth. Regu ...