western-blot

1. Load gel in apparatus as described by manufacturer. 2. Transfer proteins according to above ensuring that the NCM is toward the positive electrode. When using the Hoefer apparatus be sure to eit ...

This protocol was developed for the BIORAD protein gel and transfer apparatus. The buffers can be used with any electrophoresis/transfer system.

GeorgiaTimes"CARBONATE WESTERN TRANSFER SOLUTIONS Dunn Anal. Biochem. 1986: 157 GeorgiaTimes"1.5L GeorgiaTime ...

GeorgiaTimes"Blotting with BSA GeorgiaTimes"Blot in 5% mile/ 1X PBST (or 1X TBST) --60'/Rt GeorgiaTimes"Wash 2X for 5' in 1XPBST GeorgiaTimes"10Ab in 1% BSA/1X PBST --60'/Rt Geor ...

1. While your SDS-PAGE gel is running make your transfer buffer and chill to 4°C. Check that you have a frozen buffer dam is ready (stored in freezer next to Shikha top shelf to the right s ...

1. Run an SDS gel as usual. (It's nice to run a lane of prestained markers which will transfer on to the blot and control for the transfer).2. Transfer: make 4 liters of transfer buffer. Cut 3 pieces ...

Western免疫印迹（Western Blot）是将蛋白质转移到膜上，然后利用抗体进行检测。对已知表达蛋白，可用相应抗体作为一抗进行检测，对新基因的表达产物，可通过融合部分的抗体检测。 一、原理 与Southern或Northern杂交方法类似，但Western Blot采用的是聚丙烯酰胺凝胶电泳，被检测物是蛋白质，“探针”是抗体，“显色”用标记的 ...

(一)蛋白质电泳 1.请参照常规电泳操作. 2.分子量标准: 可选择普通分子量标准或预染的分子量标准.在选择预染的分子量标准时要注意其优点和缺点: 优点: 在电泳过程中可监控蛋白质分离状态; 可指示转印中蛋白质的转印效率. 缺点: 染料影响蛋白质移动速度;分子量测定略欠精确;影响蛋白质与膜的结合力. 购买信息: 中范 ...

Cell Lysis/Western/IP Steven FinkbeinerDepartments of Neurology and PhysiologyUCSF http://gweb1.ucsf.edu/labs/finkbeiner/Protocol/protocol_list/Cell_Lysis.shtml compiled by 6/95 by AJS from AB)Fresh 1 ...

1)Run protein gel. Set up blot: 2)Prepare two tupperware containersone with dH2O and one with 1/2X Transfer Buffer (TB). 3)Remove one glass plate from the gel by twisting a spacer between the plates.T ...

Western免疫印迹（Western Blot）是将蛋白质转移到膜上，然后利用抗体进行检测。对已知表达蛋白，可用相应抗体作为一抗进行检测，对新基因的表达产物，可通过融合部分的抗体检测。 本文主要通过以下几个方面来详细地介绍一下Western Blot技术: 一、原理 二、分类 i.放射自显影 ii.底物化学发光ECL iii.底物荧光ECF iv.底物DAB呈色 三、主要试剂 四、主要步骤 五、 ...

蛋白质的检测与分析――Western blot 操作步骤 一个基因表达终极结果是产生相应的蛋白质（或酶）。因此检测蛋白质是测定基因表达的主要标志，检测蛋白质的方法很多，除ELISA法外，也可用与检测DNA和RNA相类似的吸印方法。前两法有“南”和“北”之意，故本法遂被延伸称为Western（西）印迹法，该法能用SDS聚丙烯酰胺凝胶电泳分辨出与专一抗血 ...

METHOD for Western Blots: 1.While your SDS-PAGE gel is runningmake your transfer buffer and chill to 4℃.Check that you have a frozen buffer dam is ready (stored in freezer next to Shikhatop shelf to t ...