DNA基础知识

Materials:phenol:chloroform (1:1)chloroformAdd an equal volume of buffer-saturated phenol:chloroform (1:1) to the DNA solution. Mix well. Most DNA solutions can be vortexed for 10 sec except for high ...

Simplified DNA Extraction from Cell or TissueAuthor: Long-Cheng LiSource: Protocol OnlineAbstract: This method doesn't require organic extraction and centrifugation. It's the most simplest way of prep ...

DNA and RNA EXTRACTIONSA protocol / method / schedule /procedure for extraction / isolation of both DNA and RNA from the same material typically plant leaf / leaves(See also DNA Isolation protocol)1) ...

DNA and RNA EXTRACTIONSA protocol / method / schedule /procedure for extraction / isolation of both DNA and RNA from the same material typically plant leaf / leaves(See also DNA Isolation protocol)1) ...

Procedure 1. Grind 2 to 5 g of frozen leaves to a very fine powder with a liquid nitrogen-cooled mortar and pestle. 2. Add 25 ml of CTAB Buffer and transfer to a 50 ml tube. 3. Incubate at 65°C for 2 ...

Procedure 1. Grind 2 to 5 g of frozen leaves to a very fine powder with a liquid nitrogen-cooled mortar and pestle. 2. Add 25 ml of CTAB Buffer and transfer to a 50 ml tube. 3. Incubate at 65°C for 2 ...

CTAB TECHNIQUE / Method / Schedule / Protocol (JPB) FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES (see also DNA RNA double isolation procedure if both DNA and RNA are needed) ...

CTAB TECHNIQUE / Method / Schedule / Protocol (JPB) FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES (see also DNA RNA double isolation procedure if both DNA and RNA are needed) ...

grow plants in trays of 96 and leave two spots open (for the PCR controls) harvest 1 to 2 young and green leaves (1cm2/plant at rosette stage if possible). Use 96 well plates (1 or 2 ml E&K polypropyl ...

Overview This protocol describes a method for isolating DNA from plant tissue. Procedure1. Preheat the CTAB Isolation Buffer at 60°C. 2. Grind 2 g of fresh leaf tissue to a powder in Liquid Nitrogen ...

Overview This protocol describes a method for isolating DNA from plant tissue. Procedure1. Preheat the CTAB Isolation Buffer at 60°C. 2. Grind 2 g of fresh leaf tissue to a powder in Liquid Nitrogen ...

Streamlined DNA Extraction ProtocolThis method is derived from a procedure developed by Toby Bradshaw and the Poplar Molecular Genetics Cooperative. We have tested the procedure with a variety of Popu ...

ProcedureGrind in mortar and pestle or Waring blender with 5-7 volumes buffer A per g tissue. Use MCE at 350 l/L and if necessary with 5 ml 1 M DIECA/L. Squeeze through cheesecloth two layers of Mirac ...

Silica Clean-up of DNAMaterials:Silica Suspension: add 2 g of silica to 15 ml of H2Owash 3x by centifugation at 2000 x g for 2 minestimate vol of silica and resuspend in 2 vol H2O Silica Wash Solution ...

ProcedureGrind in mortar and pestle or Waring blender with 5-7 volumes buffer A per g tissue. Use MCE at 350 l/L and if necessary with 5 ml 1 M DIECA/L. Squeeze through cheesecloth two layers of Mirac ...

Phenol/Chloroform Precipitation of DNA1. Add an equal volume (equal to sample volume) of P/C to sample.2. Mix (shake don't vortex).3. Take aqueous (upper) layer. (If dirty sample repeat Ph/Chl step un ...

Gel Shift or Band Shift Assay or Electrophoretic Mobility Shift Assay (EMSA) is a technique for studying gene regulation and determining protein:DNA interactions. The assay is based on the observation ...

BAND-SHIFTThe procedure described in this chapter for the determination of affinity constants and kinetic dissociation constants by band-shift assay refers to an ideal antibody fragment (e.g. a scFv o ...

AbstractGene expression and regulation are mediated by DNA sequences in most instances directly upstream to the coding sequences by recruiting transcription factors regulators and a RNA polymerase in ...

EMSA using ds OligonucleotidesSolutions10X Annealing Buffer200 mM Tris 8.0 200 ml 1M Tris pH 8.010 mM EDTA 8.0 20 ml .5M EDTA pH 8.0500 mM NaCl 100 ml 5M NaCl280 ml Qstore at room temperature10X Kl ...