数据库

实验试剂 1. 使用前，将RNase A全部加入Solution I中并于4度保存。2. 按下表用无水乙醇稀释DNA Wash Buffer，并于室温保存。 D6926-00B: 加入60ml无水乙醇 D6926-01B: 加入160ml无水乙醇 D6926-03B: 加入200ml无水乙醇 D6926-04B: 每瓶中加入200ml无水乙醇 实验步骤 ...

实验试剂 20x Saline-sodium citrate buffer Details：SSC: 3 M NaCl 0.3 M sodium citrate pH 7 or Product No. S6639. RNase A Details：Product No. R4642 100 µg/ml in 2x SSC. Pepsin Details：Product No. P6887 ...

实验试剂 1. Absolute ethanol (96%-100%) - Do not use other alcohols 2. 2-mercaptoethanol 实验设备 1. Tabletop micro-centrifuge and nuclease-free 1.5 ml tubes 2. Water bath set to 30°C 实验步骤 1. Ino ...

实验原理 氯化三苯基四氮唑（TTC）是标准氧化电位为80mV的氧化还原色素，溶于水中成为无色溶液，但还原后即生成红色而不溶于水的三苯甲瓒，生成的三苯甲瓒比较稳定，不会被空气中的氧自动氧化，所以TTC被广泛地用作酶试验的氢受体，植物根系中脱氢酶所引起的TTC还原，可因加入琥珀酸，延胡索酸，苹果酸得到增强，而被丙二酸、碘乙酸所抑制。所以TTC还原量能表示脱氢酶活性并作为根系活力的指标。 实 ...

实验试剂 1. Optional: Sterile deionized water 2. Absolute ethanol (~ 96-100%) 实验设备 1. Microcentrifuge capable of at least 13000x g 2. Nuclease-free 1.5ml centrifuge tubes 实验步骤 1. Perform agar ...

实验试剂 ProtoArray® Human Protein Microarray v5.0Blocking Buffer and Washing Buffer 10×Synthetic Block 详细信息：InvitrogenAlexa Fluor® 647 Goat Anti-Human IgG详细信息：InvitrogenForceps and deionized water ...

实验试剂 1. 聚苯乙烯塑料微量组织培养板：4×10孔。2. 包被液(pH9.6)：NaHCO3 29.3g、Na2CO3 19.5g，蒸馏水加至1000ml，置4℃保存。3. 洗涤液(0.01mol/L、pH7.4PBS)：NaCl 8.0g、KH2PO4 0.2g、Na2HPO4•12 H2O 2.9g、KCl 0.2g、Tween20 0.5ml，加蒸馏水至1000ml。4. 保 ...

实验试剂 DNA提取缓冲液(100 mmol/L Tris 100 mmol/L EDTA 100 mmol/L Na3PO4 1.5 M NaCl pH 8.0)溶菌酶(50 mg/mL)蛋白酶K(20 mg/mL)10% SDSRNAse 20 mg/mL聚乙烯基吡咯烷酮(PVP)交联聚乙烯基吡咯烷酮(PVPP)TENP缓冲液(50 mmol/L Tris 20 mmol/L EDTA 10 ...

实验试剂 试剂配制：1 .1 M Tris.Cl(pH 7.5)(100 ml)：12.1g Tris碱，加80 ml ddH2O，浓HCl调pH 7.5，定容至100 ml；2. 0.5 M EDTA(pH 8.0) (100 ml)：18.6g EDTANa2.2H2O， 70 ml ddH2O，NaOH调pH 8.0，定容至100 ml；3. 1xTE(pH 7.5) (100 ml)：1 ...

实验步骤 1.Harvest cells and wash twice with ice-cold PBS. PBS and subsequent buffers can be supplemented with 5 mM sodium butyrate to retain levels of histone acetylation.2.Resuspend cells in Triton E ...

实验步骤 1.Pellet cells2.Lyse cells in 0.5 ml detergent buffer: 10 mm Tris (pH 7.4) 5 mm EDTA 0.2% Triton 3.Vortex 4.Incubate on ice for 30 min.5.Centrifuge at 27000 g for 30 minutes 6.Divide super ...

实验原理 Principle of the methodThe acetoxymethyl (AM) ester derivatives of fluorescent indicators make up one of the most useful groups of compounds for the study of live cells. Modification of carboxyl ...

实验试剂 MaterialAmountStorageStabilityDAPI dihydrochloride (MW = 350.3)10 mgRoom temperatureProtect from lightWhen stored as directed product solids should be stable for at least 1 year. DAPI dilactate ...

实验材料 大鼠或小鼠垫料： Aspen，half Litaspen Premium 6 and half Litaspen Premium 8/20纸毛和杨木8/20 羊毛喷洒消毒剂纸羊毛和Aspen羊毛餐具洗涤剂，清洗塑料Marlau™笼（保持无味） 实验步骤 Marlau™笼设置定时7-10分钟。1．使用两个手柄将笼子放置在一个平坦的地面中，含水槽的一侧应面对你（图）。 2．插入塑 ...

实验步骤 1. Isolate a single colony from a freshly streaked selective plate and inoculate a starter culture of 2-5 ml LB or YT medium containing the appropriate selective antibiotic. Incubate for ~ 20-24 ...

实验步骤 Note: Always use less starting material (1. Disrupt the tissue and homogenize lysate in 6.0mL TRK lysis buffer with a rotorstator homogenizer. Transfer the homogenate to a 50ml centrifuge tube w ...

实验步骤 1. Tomato Seedling Growth in Pots and Trays 1) For pot assays plant tomato seeds in a single pot in an organic-rich soil such as Sunshine mix. Maintain in a greenhouse at 22-28°C. After ...

实验试剂 PBS(Gibco-Invitrogen；cat. no. 14190)accutase(PAA；cat no. L11-007)DMEM培养液(Gibco-Invitrogen；cat.no.41965-039)用于B16 - F10细胞的培养RPMI 1640(Gibco-Invitrogen；cat.no. A10491-01)用于 L1C2细胞的培养胎牛血清(PAA；cat.n ...

实验试剂 General example using DMEM media1. 450 ml DMEM - Remove 50 ml

实验试剂 TENP缓冲液(50 mmol/L Tris 20 mmol/L EDTA 100 mmol/L NaCl 1% PVP pH 10.0)PBS缓冲液(137 mmol/L NaCl 2.7 mmol/L KCl 10 mmol/L Na2HPO4 2 mmol/L KH2PO4 pH 7.4)DNA提取缓冲液(100 mmol/L Tris 100 mmol/L EDTA 100 m ...