实验材料 1. 供试植物:番茄、黄瓜、甘蓝、烟草 2. 供试昆虫:丽蚜小蜂、烟粉虱 实验步骤 1. 昆虫培育 (1) 在室内用网罩培育寄主植物,当苗高达30 cm左右时,按每叶片上5 头的比例接入粉虱成虫; (2) 接种48~72 h 后轻轻摇动植株,以赶走大部分成虫; (3) 移入到塑料罩中,用吸有敌敌畏原液2~3 滴的滤纸熏蒸10~12 h; (4) ...
实验设备 37°C water bath37°C / 5% CO2 humidified incubatorOrbital shaker placed inside incubator 实验步骤 1.Prepare compound stocks: test articles (TA) and positive control(s) (PC) dissolved in an or ...
实验原理 1 mol的己糖经过酶反应,最终转化称6-Phosphogluconat均伴随着1 mo1NADPH的产生,通过测定NADPH在340nm的光吸收,即可测定样品中的己糖含量。 实验试剂 1. 溶液的配制1mM glucose1mM fructose1mM sucrose500mM TRA buffer pH 7.4 TRA=triethanol amino2M MgS ...
实验原理 This assay is a Sandwich ELISA based sequentially on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-G ...
实验原理 血液的组成是: 血浆:优良溶剂,含蛋白质、葡萄糖、无机盐、代谢产物、O2、CO2血液 红细胞:含血红蛋白,容易和O2和CO2结合 血细胞 白细胞:具免疫(淋巴细胞),吞噬机能 血小板:止血,凝血血型:指红细胞上所含抗原的不同,应用抗原与抗体的反应,产生凝集与否,对血型进行判断:A型:红细胞上具有A抗原,能与特异性抗体A发 ...
实验试剂 Regents to be provided by user 1. Absolute (96%-100%) ethanol 实验设备 Equipments to be provided by user1. Centrifuge capable of at least 3000-5000 x g2. Rotor adapter for 96-well microplate3. ...
实验步骤 1. Protocol using XCell SureLock™ Mini-Cell1) Remove the Novex® Pre-Cast Gel from the pouch.2) Rinse the gel cassette with deionized water. Peel off the tape from the bottom ...
实验步骤 1. Resuspend the cells in standard cell culture medium and then add 10% (v/v) AlgiMatrix™ Firming Buffer to this suspension (i.e. 1 part Firming Buffer to 9 parts cells plus medium). The ...
实验试剂 1. 0.026mol/L EGTA(ethylene glycolbisαaminoethyltetraacetic acid)溶液,pH值7.5;2. 醋酸盐缓冲液,pH值7.0、0.02mol/L,含有0.75mol/L NaCl和0.01mol/LEDTA;3. pH值5.0、0.06mol/L EDTA;4. pH值5.0、0.005mol/L PB,含0.75m ...
实验步骤 1.Preparing the Glass plates1)Locate two LiCor plates (one with a notch and one without) and clean them as follows:a.Scrape off the polyacrylamide with a razor bladeb.Scrub both sides of the ...
实验原理 蛋白质是机体内主要的含氮物质,而且氮元素的含量相当恒定,一般为16%,其它非蛋白质的含氮化合物所占的氮量甚微。因此,测定生物样品的含氮量,即可推算蛋白质含量。 测定生物样品中的含氮量,最常用的方法是凯氏定氮法,本实验采用改良微量定氮法,其原理是:1. 氧化并固定有机氮质(消化):以强氧化剂(浓硫酸,亚硒酸)与稀释血清混合加热、消化,血清中的有机物质全部分解 ...
实验试剂 ChargeSwitch® Lysis Buffer (L12) Proteinase K ChargeSwitch® Magnetic Beads ChargeSwitch® Purification Buffer (N5) ChargeSwitch® Wash Buffer (W12) ChargeSwitch® Elution Buffer (E5) or TE Buffer ...
实验材料 Contents and storage information. MaterialAmountConcentrationStoragundefinedStabilityVybrant® DyeCycle™ Violet stain200 μL5 mM solution in deionized water2-6?CDO NOT FREEZEProtect from lightWhen ...
实验步骤 1. 将保存在2.5%重铬酸钾中的柔嫩艾美耳球虫孢子化卵囊悬液离心10min(3000r/min),弃去上清,再用PBS重悬沉淀,重复洗涤2次,除去重铬酸钾;2. 经口接种给饲养于无球虫环境下7日龄的小鸡,攻虫数量为孢子化卵囊5×104个/只,感染后第8天扑杀所有攻毒的鸡只,解剖收取盲肠;3. 收集的盲肠经过匀浆机匀浆,加入清水3000rpm离心15min,弃上清,用适当PBS重悬;4 ...
实验试剂 Water distilled Hanks’ Balanced Salt Solution (HBSS) without Ca2 and Mg2 Dulbecco’s Phosphate-Buffered Saline (D-PBS) without Ca2 and Mg2 D-Glucose Penicillin-Streptomycin Ascorbic Acid StemP ...
实验试剂 L-Leucine,ATP,DTT,焦磷酸钠(tetrasodium pyrophosphate)和HA-Ultrogel购自美国Sigma公司。p-焦磷酸钠购自美国杜邦公司。L-C-亮氨酸和DEAE-SepharoseTM Fast Flow购自英国Amersham公司。限制性内切酶和T4DNA连接酶购自立陶宛MBI Ferments公司。 实验材料 质粒pTrc-99 ...
实验试剂 FA Neutralization Solution(1M Tris base 0.5 M Na2HPO4 0.05% NaN3)1.60.57 g Tris base2.35.50 g Na2HPO43.2.5 ml 10% NaN34.H2O to 500 ml; pH is not adjusted; store and use at room temperature.5 ...
实验试剂 1. Buffer A 10mM HEPES 1.5mM MgCl2 10mM KCl 0.5mM DTT 0.05% NP40 (or 0.05% Igepal or Tergitol) pH 7.92. To prepare 250ml stock of Buffer A: HEPES: 1M = 238.3g/litre ...
实验原理 MISSION® TurboGFP™ control particles enable fast determination of the appropriate transduction conditions for efficient transduction of cells with lentiviral particles. Note: The CMV promoter is ...
实验试剂 Regents to be provided by user 1. Absolute (96%-100%) ethanol 实验设备 Equipments to be provided by user1. Centrifuge capable of 4000 x g.2. 96 well Plate3. Water bath preset at 65-70°C 4. Equi ...