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Abstract Table of Contents Materials Figures Literature Cited Abstract The generation of transgenic mice by DNA microinjection is a powerful tool to investigate the molecular regulation of gene expression

Abstract Table of Contents Materials Figures Literature Cited Abstract Colorectal distension (CRD) is a widely accepted, reproducible method for assessing visceral sensitivity in both clinical and pre?cli

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes the testing of rats in prime?, footshock?, and cue?induced reinstatement procedures. Evaluating rats in these pro

Abstract Table of Contents Materials Figures Literature Cited Abstract Engineered transcription activator?like effector nucleases (TALENs) are broadly useful tools for performing targeted genome editing i

Abstract Table of Contents Materials Figures Literature Cited Abstract Re?formation or preservation of functional, electrically active neural networks has been proffered as one of the goals of stem cell?m

Abstract Table of Contents Materials Literature Cited Abstract This unit describes a robust protocol for producing multipotent Kdr?expressing mesoderm progenitor cells in serum?free conditions, and for functional genomics screening using t

Abstract Table of Contents Materials Figures Literature Cited Abstract Human embryonic stem cells (hESC) are difficult to adapt to 96?well plate assays, such as the MTT assay, because they survive best wh

Abstract Table of Contents Literature Cited Abstract Laboratory models that accurately replicate human tumor initiation and characteristics are integral to advancing knowledge in cancer research. However, comparative studies between commonly employed laboratory mod ...

Abstract Table of Contents Materials Figures Literature Cited Abstract The water maze task is widely used to evaluate spatial learning and memory in rodents. The basic paradigm requires an animal to swim

Abstract Table of Contents Materials Figures Literature Cited Abstract Gene targeting by sequence?specific nucleases in one?cell embryos provides an expedited mutagenesis approach in rodents. This technol

Abstract Table of Contents Materials Figures Literature Cited Abstract The availability of high?throughput biochemical and imaging techniques that can be used on live mice has increased the possibility of

Abstract Table of Contents Materials Figures Literature Cited Abstract Morbid obesity is linked to increased incidence of glucose intolerance, Type 2 diabetes mellitus, cardiovascular diseases, various fo

Abstract Table of Contents Materials Figures Literature Cited Abstract Atherosclerosis is considered a chronic inflammatory condition of the vessel wall and involves a high chronic concentration of low?de

Abstract Table of Contents Materials Figures Literature Cited Abstract The normal gut microbiota has evoked many investigators' interest over the years and the pioneering work of James Reyniers in the 192

Abstract Table of Contents Materials Figures Literature Cited Abstract The response of respiratory tract tissue target sites to inhaled vapors depends on the amount of inhaled vapor that is delivered to t

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes two basic protocols for the detection of the proliferation of neural stem cells (NSC). The first one addresses ce

Abstract Table of Contents Materials Figures Literature Cited Abstract Research models that replicate the diverse genetic and molecular landscape of breast cancer are critical for developing the next?gene

Abstract Table of Contents Materials Figures Literature Cited Abstract Chronic obstructive pulmonary disease (COPD) is characterized by progressive airflow limitation resulting from inflammation?driven pa

Abstract Table of Contents Materials Figures Literature Cited Abstract Human asthma is defined as a chronic inflammatory disease of the airways. Animal models are required to study asthma pathophysiology

Abstract Table of Contents Figures Literature Cited Abstract Type 1 diabetes (T1D) is characterized by a partial or total insufficiency of insulin. The premiere animal model of autoimmune T cell?mediated T1D is the NOD mouse. A dominant ne