Researchers are usually trained in RNA isolation and analysis methods by one another or by technical manuals. Experimental procedures are often not questioned and quickly become dogma. Furthermore it ...
RNA quantitation is an important and necessary step prior to most RNA analysis methods. Here we discuss three common methods used to quantitate RNA and tips for optimizing each of these methods. UV Sp ...
目的为动物来源性产品中150多种兽药残留的筛查提供一种有效的解决方案。前言兽药广泛的应用于治疗或预防动物疾病，但会带来肉、鱼、奶、蛋和蜂蜜等动物性产品中的痕量药物残留。由于对人体健康存在潜在的危害，食品链中药物残留的存在也引起了广泛的关注。确实，污染的动物产品可能导致过敏反应或由于细菌抵抗的发展导致临床治疗上间接的问题。为了保护消费者的健康并确保动物性产品的质量，在世界范围内设立了动物产品中药物允 ...
一、琼脂糖凝胶的特点天然琼脂（agar）是一种多聚糖，主要由琼脂糖（agarose，约占80％）及琼脂胶（agaropectin）组成。琼脂糖是由半乳糖及其衍生物构成的中性物质，不带电荷，而琼脂胶是一种含硫酸根和羧基的强酸性多糖，由于这些基团带有电荷，在电场作用下能产生较强的电渗现象，加之硫酸根可与某些蛋白质作用而影响电泳速度及分离效果。因此，目前多用琼脂糖为电泳支持物进行平板电泳，其优点 ...
Units1 mg = 10-3 g1 ug = 10-6 g1 ng = 10-9 g1 pg = 10-12 g 1 kb of double stranded DNA = 660 kD 1 kb of single stranded DNA = 330 kD 1 kb of single stranded RNA = 340 kD Average MW of a deoxynucleotid ...
Purpose and backgroundsPrinciple of radioiodinationAddition of oxidizing reagents (such as chloramine-T or peroxidase+H202) converts I- to I+ or I3-. This highly reactive molecule attacks o-position o ...
In the early eighties a series of papers were published by Gorm DANSCHER Aarhus Denmark to introduce a reliable and easy-to-handle technique for light microscopical and ultrastructural autometallograp ...
细胞培养器材的选择：从大小来讲，细胞培养瓶约可分为约600ml，250ml，50ml和25ml的，一般都经过表面改性处理，适合细胞贴壁和生长。600ml 、300ml的多用于大规模培养时用（如单克隆细胞的培养等），50ml的多用于一般性细胞实验用（一般性的传代，保存细胞，为实验提供细胞等），25ml一般是用来复苏细胞或细胞较少时的培养，还有做原代细胞时也可以做多瓶而避免交叉污染。当然还有其他如圆形 ...
A few cell types are thin enough to be viewed directly in a microscope (algae protozoa blood tissue cultures) but most tissues (kidney liver brain) are too thick to allow light to be transmitted throu ...
Large Scale Preps: (See Large scale plsasmid prep protocol for more details) Cultures: Inoculate a 5 mL LB/Amp (50 - 100 µg/mL) culture in early a.m. with a single colony. Use all 5 mL to inoculate a ...
Materials: 250 µL of fresh mouse blood in plastic tubes containing EDTA. RBC lysis buffer (388 mM NH4Cl 29.7 mM NaHCO3 25 µM Na2EDTA) 20.75g. NH4CL2.5g NaHCO30.093g Na2EDTA1L H2OHemocytometer Hema ...
1. Simple Dilution (Dilution Factor Method based on ratios)A simple dilution is one in which a unit volume of a liquid material of interest is combined with an appropriate volume of a solvent liquid t ...
Antibiotics should be added to lower than 60 C broth or filter sterilized: See note. "C'' refers to chromosomal resistance: "P" plasmid based resistance "FS" filter sterilization required. Temperature ...
A. Storage . The following properties of reagents and conditions are important considerations in processing and storing DNA and RNA. Heavy metals promote phosphodiester breakage. EDTA is an excellent ...
This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison. The ups and downs of various methods from sonica ...
Jackson ImmunoResearch 公司二抗选用指南(此部分内容来源于Jackson ImmunoResearch Laboratories Inc网站，如有疑问，请参考原网站）亲和层析法纯化的二抗 用亲和层析法纯化的抗体是指，利用耦联到琼脂糖凝胶上的抗原将抗血清中的抗体亲和层析下来。我们采用一种特有的连续的洗脱过程将抗体从固态抗原上分离出来。我们提供的未标记的亲和层析法纯化的抗体是不含 ...
用液相色谱法测定甲苯二异酸酯异构体含量.pdf土壤中有机氯的前处理及分析方法.doc微波辅助萃取-GC-MS联用快速分析.doc ...
医用低速冷冻离心机的常见故障与处理方法 离心机是利用离心力分离液体与固体颗粒或液体与液体的混合物中各组分的机械，应用于医学中的离心机就叫做医用离心机。医用离心机根据最大转速可分为低速离心机（30 000 r/min)，按有无制冷设备可分为冷冻离心机和普通离心机。现把多年来在设备维修中遇到的冷冻离心机的故障现象和维修方法以低速冷冻离心机为例进行总结。例:低速冷冻离心机是最高转速为4000 r/min ...
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