实验仪器使用技术

Asymmetric sequences are indicated by *.Single letter code:R = G or A;Y = C or T;W = A or T;M = A or C;K = G or T;S = C or G;H = A C or T;V = A C or G;B = C G or T;D = A G or T;N = G A T or C.Re ...

Single letter code:R = G or A;Y = C or T;W = A or T;M = A or C;K = G or T;S = C or G;H = A C or T;V = A C or G;B = C G or T;D = A G or T;N = G A T or C.RecognitionsequenceEnzyme^(10/15)AC(N)4GTA ...

是目前最全的实验方法的站点，有许多实验中的疑难问题的解决方法。http://www.bioon.net/biotech.htm收集了最经典的protocol网站，大家多留心点！Such as:(1)iprotocol：http://iprotocol.mit.edu/有近三百种方法，涉及Animal Studies、Biochemistry、Bioengineering、Bioinformatic ...

生物碱类（Alkaloids） 是存在于生物体（主要为植物）中的一类含氮的碱性有机化合物，大多数有复杂的环状结构，氮素多包含在环内，有显著的生物活性，是中草药中重要的有效成分之一。如黄连中的小檗碱（黄连素）、麻黄中的麻黄碱、萝芙术中的利血平、喜树中的喜树碱、长春花中的长春新碱等。 含生物碱的中草药很多，如三尖杉、麻黄、黄连、乌头、延胡索、粉防已、颠茄、洋金花、萝芙木、贝母、槟榔、百部等，分布于10 ...

腺病毒载体的优点：1. 宿主范围广 对人致病性低腺病毒载体系统可广泛用于人类及非人类蛋白的表达。腺病毒可感染一系列哺乳动物细胞，因此在大多哺乳动物细胞和组织中均可用来表达重组蛋白。特别需要指出的是：腺病毒具有嗜上皮细胞性，而人类的大多数的肿瘤就是上皮细胞来源的。另外，腺病毒的复制基因和致病基因均已相当清楚，在人群中早已流行（70-80%成人体内都有腺病毒的中和抗体存在）。人类感染野生型腺病毒后仅产 ...

Acetone / Formaldehyde Fixative Acrylamide Gel denaturing Acrylamide Gel nondenaturing Alkaline Phosphatase Buffer 1 (Glycine Buffer 0.1 M pH 10.4) Alkaline Phosphatase Buffer 2 (Tris-HCl 100 mM pH 9. ...

Antibiotic Concentration in MediaAntibiotics should be added to lower than 60 C broth or filter sterilized: See note. "C' refers to chromosomal resistance: "P" plasmid based resistance "FS" filter ste ...

PHEM (500 mls) 2x 18.14 g Pipes 6.5 g Hepes 3.8 g EGTA 0.99 g MgSO4 pH 7.0 w/ KOHPBS (5x in 500 mls) 20.45 g NaCl 0.465 g KCl 10.142 g Na2HPOundefined7 H2O 0.545 g KH2PO4 pH 7.2Mounting Media 20mM Tris pH 8. ...

Sterile water bottles 200 ml 500 ml or 950 ml water.Autoclave.0.05 M CaCl2 (per 200 ml) CaCl2・2 H2O 1.5 gAdd 198 ml water.Autoclave.0.5 M CaCl2 (per 200 ml) CaCl2・2 H2O 15.0 gAdd 188 ml water.Autoclav ...

Phosphate (Sodium) buffer ChartStock solution A 2 M monobasic sodium phosphate monohydrate (276g/L) Stock solution B 2 M dibasic sodium phosphate (284 g/L). Mixing an appropriate volume (ml) of A and ...

Working concentrations and stock solutionsPreparation of stock solutionsAmpicillinCarbenicillinGE antibiotic mixTetracyclineChloramphenicolSupplement.About the recalculation of recipes for the arbitr ...

TE 1x 10xSTE 1x 10xSSC 20xElectrophoresis buffers.TAE 50xTBE 10xFGRB 50xGTGB 20xTGB 5xLoading buffer 10xPEG-NaClBacteriophage lambda buffersSMSTMTMDenaturation bufferNeutraliszation bufferDeveloper Re ...

1. Simple Dilution (Dilution Factor Method)A simple dilution is one in which a unit volume of a liquid material of interest is combined with an appropriate volume of a solvent liquid to achieve the de ...

08-15 Buffer CompoundMWFinal concentration200 mlGlycine75.070.1 M1.5 g1 M MgCl210 mM2 mlKOHto pH 9H2Oto 200 mlA Acetic Acid 1 M CompoundMWFinal concentration50 mlAcetic Acid 98% (~17 M)1 M3 ml ...

AcO---- part one ---(AcO)2Ca 1; 1.5MAcOK 1; 2; 3; 4; 5; 6; 7; 8M(AcO)2Mg 1MAcONa 1; 2; 3; 4MpH of Ac K/Na solutionsAcONH4 1; 2; 3; 4MCO3-K2CO3 1; 2; 3; 4; 5; 6MKHCO3 1MNa2CO3 1; 2MNaHCO3 1M( ...

Organic substances.pKa and temperature dependence of pH for common buffers.ATP 0.1MBetaine 5MCresol red (Na) 50mMDTT 1M 2.2MdNTP’s 100mMEDTA 0.5MEtBr 10mg/mlGelatin 2%Glucose 1; 1.5; 2MGuanidine HCl 1 ...

The following is a list of the solutions and chemicals required throughout the laboratory manual. It is organized alphabetically and individual exercises list the materials needed for that exercise. F ...

Turn on machine and wait for it to warm up Wash glass cuvettes thoroughly before use and if 2 are used then make sure they are a ‘matching’ pair. For Spec. in Dave Andersons bay then press ‘goto ?’ ty ...

The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie Brilliant blue to protein.There is no interference from cations ...