实验仪器使用技术

Alkaline Phosphatase for Glycol Methacrylate SectionsProcedure:Incubate sections in the incubating medium at room temperature for 1 - 3 hours. Two hours is sufficient in most cases. Wash in distilled ...

Glycol Methacrylate Embedding for ImmunohistochemistryTechnovit Glycol Methacrylate: Technical Information and Help From Heraeus Kulzer.Embedding in plastic provides many advantages to the histotechno ...

Glycol Methacylate Embedding for Materials SamplesTechnovit Glycol Methacrylate: Technical Information and Help From Heraeus Kulzer.Embedding in a plastic often supplies the support necessary to succe ...

Staining Protocols for Lymphoid Tissue:Technovit Glycol Methacrylate: Technical Information and Help From Heraeus Kulzer.Normal immunostaining procedures for routine markers on sections obtained from ...

COMBINED ALCIAN BLUE - P.A.S SECTIONS4micron paraffin wax sectionsSOLUTIONS1. Alcian Blue pH 2.5Alcian Blue 1g3% Acetic Acid 100ml2. 1% Periodic Acid 3. Schiffs ReagentMETHODTake sections to waterPlac ...

Periodic Acid Schiff Stain for Glycol Methacrylate Sections Technovit Glycol Methacrylate: Technical Information and Help From Heraeus Kulzer.Procedure: Oxidize sections in 0.4% periodic acid for 30 m ...

Sectioning and Mounting ProtocolTechnovit Glycol Methacrylate: Technical Information and Help From Heraeus Kulzer. SectioningSectioning is best done with a rotary or sledge microtome such as the JB-4 ...

for the technique of embedding specimens in plasticDay 1Step 1: Read and understand the Material Safety Data Sheets associated with each of the chemicals used in this procedure. Use of rubber gloves l ...

1.Deparaffinize slides a (after drying thoroughly overnight at RT) in 2 changes of xylene (or xylene substitute) for 10 mins each. 2.Transfer slides to 100% alcohol 2 changes for at least 2 m ...

Preparation and Staining of Paraffin Sectionsfor use with Pharmingen reagents I. Fixation and Processing of Tissue for Paraffin SectionsA. Fixation of Tissues in 10% Neutral Buffered FormalinSacrifice ...

Preparation and Staining of Frozen Tissue Sectionsfor use with Pharmingen reagents I. Preparation of Frozen Sections for SectioningMaterials neeed:2-methylbutane (isopentane) Liquid Nitrogen Dry ice P ...

SECTION ADHESIVES It is advisable to use an adhesive to promote tissue attachment to the glass slides used in histological preparation. This is routinely achieved by the application of a smear of glyc ...

Material MEMPfa: 0.1M MOPS -- pH 7.4 2mM EGTA 1mM MgSO4 (typically this is made up as 10X MSalts. 4% paraformaldehyde (diluted from a frozen stock of 20%) (store MEMPfa at 4C and use within 1 day). ...

CryosectioningWhile the timing of the various steps in this protocol are probably not critical I tend to prefer to process the tissue all at once to ensure that RNA and/or proteins do not get degrade ...

All tissue types except muscle:Tissue should preferably be snap-frozen by quenching in liquid nitrogen. Quenching is accomplished by putting a small amount of OCT compound onto a cork disc placing the ...

All tissue types except muscle:Tissue should preferably be snap-frozen by quenching in liquid nitrogen. Quenching is accomplished by putting a small amount of OCT compound onto a cork disc placing the ...

Hematoxylin StainSolutionsHematoxylinHematoxylin6.4gAmmonium alum60gEthanol200mlGlycerol160mlDistilled water640mlMix for a day then allow the solution to ripen in the dark for at least a month before ...

ACETYL CHOLINESTERASE SECTIONS Unfixed cryostat or cold Formol calcium/gum sucrose fixed sections. SOLUTIONS a. Incubating solution. --acetyl thiocholine iodide 5mg --0.1M acetate buffer (pH.6.0) 6.5m ...

ACID PHOSPHATASE Sections 5-10 micron unfixed or cold formol calcium/gum sucrose fixed Cryostat sections.Stock solutionsA) Pararosanilin-HCL StockPararosanilin 1gDistilled water 20mlHCl Conc 5mlThe pa ...

Stain for Sperm Acrosomes Procedure 1. After sperm preparation adjust sperm concentration as high as possible (5-10 x 106/ml). 2. Prepare a smear of sperm suspention onto a slide and allow it to dry. ...