Bovine Rectal PalpationAnatomical Basis of Rectal Palpation Manipulation and examination of the reproductive tract of cattle via rectal palpation is possible because of several anatomical features of the cow:1) As in other species, the reproductive tract of the bovine is suspended f ...
The FAM caspase binding assay kits from ATCC Corporation can be used to determine amounts of active caspases in cells. The FAM-labeled caspase inhibitor can freely diffuse into the cell. Active caspase irreversibly binds the inhibitor. Upon washing the cells, the amount of fluorescence is pr ...
Differential staining of trophectoderm and inner cell mass in combination with TUNEL labeling of bovine embryos将滋养层和内细胞群分别染色，TUNEL检测牛胚胎凋亡Simple protocol for differential staining/TUNEL analysis of bovine embryosThe procedure for differential staining of troph ...
Making collagen solution from lyophilized rat tail collagen鼠尾制备胶原蛋白Chen-Ming Fan Lab,Carnegie Institute of Washington(lyophilized rat tail collagen from Roche)DME power from Life technologies (buy the one withoutNaHCO3, make a 10X solution but with the pH color indicator, an ...
Genotyping from mouse tailsChen-Ming Fan Lab,Carnegie Institute of Washington(Laura’s method)All pipets must be filter tippedBe very careful to prevent cross contamination of samplesChange gloves oftenDo not vortex the tubes1. Place tail tip in 500 uL of Transgenic lysis buffer (m ...
Embedding, Sectioning, and Mounting of Frozen TissuesChen-Ming Fan Lab,Carnegie Institute of Washington Embedding of Frozen Tissues.Let PFA fixed embryos sink to the bottom of a tube containing a 30% sucrose (inPBS) solution overnight. After they have sunk, remove all but 2-3 mL's of thesol ...
Banker Culture Hippocampal Dissection 1) Begin by placing two large (100mm) and three small (60mm) petri dishes in an ice bath. Fill with cold HBSS medium (calcium and magnesium free). Place a large Falcon tube (50ml) containing fresh HBSS in bath.2) Euthanize the pregnant rat with halothane by plac ...
Adenovirus Cre Intranasal Infection Information & Protocol腺病毒鼻内感染小鼠 Jacks Lab, Center for cancer research,MIT http://web.mit.edu/ccr/labs/jacks/protocols/AdenovirusCre.htmlREAGENTS: MEM (Sigma catalog #M-0268) 2 M CaCl2 *Adenovirus - University of Iowa Notundefined- The U ...
Principles of SurgeryWelcome to the Principles of Surgery Home Page . The goal of this project is to aid students in mastering the skills and information presented in the course 'Principles of Surgery'. the principles of surgery home page is below:http://cal.vet.upenn.edu/surgery/ 上一篇：急性 ...
Procedures Common to the First Five Behavioral TestsMarking the miceOn Monday morning in the colony room, tail mark the 32 mice to be tested in the AM. Use a Sharpie pen (waterproof black or red) to make 1 or 2 tail bands near the base of the tail. Start at cage 1 and proceed in order to cage 16. Capture one mouse, then consult a l ...
Features of the Husbandry to be Equated Across LabsFive periods may differ in the requirements to some extent. Features mentioned only for the first period are common to all periods.After arrival from supplier but before mating; housed with same sexMated pairs before and after delivery of lit ...
Quick and simple mouse forebrain commissure anatomy1. Make solutions ahead of time2. Euthanasia - use either CO2 or pentobarbital sodium; consult a veterinarian3. Rapidly remove the brain from the skull.4. Fix the brain in 10% formalin or 4% paraformaldehyde for at least 24 hours.5. Trim and weigh ...
Protocol for Rotarod Coordination testingApparatus 2 SmartRods from Accuscan. Each box has a plastic bedding tray added that is about 2 cm above the photocell beams so that the mouse can never break the photocell beams. The tray should be filled with about 1cm of the standard 1/4" bed-o-cob bedding. The ...
Locomotor Activity Testing ProtocolApparatus and Room Setup1. Activity monitors are enclosed in a sound attenuating chamber with a ventilation fan on. Fan provides some masking noise. Fan is a common 3" or 4" muffin fan (110 VAC). We can try to define the actual sound level, but matching may not be easy sin ...
Protocol for Elevated Plus MazeBasic FeaturesThe test will be a five min test on the maze immediately following transport from the holding area in the home cage.General Apparatus Setup and Room Configuration1. Mazes are to be placed in an area where as much symmetry as possible is achieved from the r ...
Water Maze Escape Learning TaskPhysicalTank (provided by DW lab)Material polypropyleneColour whiteDiameter 70 cm insideHeight 20 cmRim L shaped, 3x3 cmWater (local tap water)Depth 15 cm; Volume =15 cm x (35 cm)2 = 57,727 cc = 57.7 LSurface to rim 5 cmTemperature 25 ± 1 COpacity For visible platfo ...
Cocaine-stimulated Locomotor Activity Testing ProtocolBackground DiscussionThis test should have the same length as the Monday activity test (15 min). Cocaine will be administered immediately prior to placement in the activity chamber.Otherwise the protocol here is the same w ...
Dissociated Cultures of Cerebellar Neurons分离培养大鼠或小鼠小脑神经元Protocol Dissection can do on counter (i.e., in non-sterile conditions)use P8 rat pups, or P5 mouse pupskeep on ice whenever possiblefor each step, do all pups, before moving to next stepfill 2 tubes with approx. 30ml HHGN (one for di ...
Investigating the behavioral displays of male Siamese fighting fish, Betta splendensIntroductionSiamese fighting fish (Suborder Anabantoidei) have long been valued by aquarists for their brilliant colors, ornate fin shapes, and stylized behavioral displays. Original ...
Alcohol ConsumptionOverall: Two days of water, followed by two, 2-day choices of 6% ethanol vs. water, with a position change between days 1-2 and 3-4.After the cocaine test of Friday, mice are placed by pairs into a new home cage (i.e., bedding change) with a water bottle that has a sipper tube (something that Po ...
关于丁香通
公司信息
个人用户
企业机构
