Imaging mass spectrometry (IMS) allows the direct investigation of both the identity and the spatial distribution of the entire molecular content directly in tissue sections, single cells, and many other biological surfaces. We describe here the steps required to retrieve the molecul ...
Characterizing the molecular contents of individual cells is critical for understanding fundamental mechanisms of biological processes. Imaging mass spectrometry (IMS) of biological systems has been steadily gaining popularity for its ability to create precise chemical i ...
Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) uses inorganic particles or porous surfaces as the energy-mediating means to promote desorption and ionization of low-mass analytes of interest. With good stability during laser ablation, SALDI su ...
Desorption electrospray ionization mass spectrometry (DESI-MS) is a relatively new ambient surface analysis method. This technique requires little or no sample preparation prior to the actual analysis. Along with other application areas, DESI-MS is used to obtain chemical images of ...
We describe the utility of intermediate-pressure MALDI and tandem mass spectrometry (MS/MS and MS n ) for the characterization and imaging of phospholipids in brain tissue sections. The use of both MS/MS spectra and MS/MS images allows for identification of isobaric compounds. The structu ...
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) has proven to be an extremely powerful tool for characterizing chemical distributions within biological cells and tissues. However, differentiating biological samples, e.g., cancerous cells from their normal count ...
Tissue profiling and imaging by MALDI mass spectrometry has allowed the direct analysis and localization of biomolecules in tissue. However, due to the in situ nature of this technique, the complexity of tissue, and the need for a chemical matrix in MALDI, the signal recorded can be extremely comp ...
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) can be utilized to map the distribution of various molecules on a surface with submicrometer resolution. Many of its biological applications have been in the study of membrane lipids, such as phospholipids and cholesterol. For ...
A detailed protocol for quantitative single cell mass spectrometry imaging (MSI) analysis is described in this chapter with examples of the treatment of cells with anticancer drug, cisplatin. Cisplatin, cis-diamminedichloridoplatinum ii (CDDP), is widely used for the treatment of ...
Matrix deposition is a critical step in tissue imaging by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). It greatly affects the quality of MALDI imaging, especially for the analytes (such as lipids) that may easily dissolve in the solvent used for the matrix a ...
Laser ablation electrospray ionization (LAESI) is a novel method for the direct imaging of biological tissues by mass spectrometry. By performing ionization in the ambient environment, this technique enables in vivo studies with potential for single-cell analysis. A unique aspect of ...
Matrix-assisted laser desorption/ionization-tandem mass spectrometric method (MALDI-MS/MS) has proven to be a reliable tool for direct measurement of the disposition of small molecules in animal tissue sections. As example, MALDI-MS/MS imaging system was employed for visuali ...
The determination of the localization of various compounds in a whole animal is valuable for many applications, including pharmaceutical absorption, distribution, metabolism, and excretion (ADME) studies and biomarker discovery. Imaging mass spectrometry is a powerful tool f ...
Significant advances have been made in the past decade in the field of mass spectrometry imaging with MALDI ion sources (MALDI-MSI). While MALDI-MSI has high potential in the field of biology and in the clinic, a challenge for MALDI-MSI has been to adapt itself to a greater range of sample types. In partic ...
Identification of potential markers of a physiological stage (e.g., pathology) discovered using MALDI-MSI is an important step in the understanding of signaling pathways or for providing sets of diagnosis and prognosis markers for clinical applications. Classically, identifi ...
MALDI imaging as a molecular mass spectrometry imaging technique (MSI) can provide accurate information about molecular composition on a surface. The last decade of MSI development has brought the technology to clinical and biomedical applications as a complementary technique of M ...
This chapter describes the utility of structurally based separations combined with imaging mass spectrometry (MS) by ion mobility-MS (IM-MS) approaches. The unique capabilities of combining rapid (μs-ms) IM separations with imaging MS are detailed for an audience ranging from new to p ...
Cancer research is one of the most promising application areas for the new technology of MALDI tissue imaging. Cancerous tissue can easily be distinguished from healthy tissue by dramatically changed metabolism, growth, and apoptotic processes. Of even higher interest is the fact that MA ...
MALDI-MSI has been demonstrated to be a suitable technique in pharmaceutical research for providing information of the distribution of low molecular weight compounds such as drugs and their metabolites within whole-body tissue sections. Important ADME information can be determ ...
The resolution of MALDI MS imaging is limited by the displacement of analytes during matrix deposition or by laser focal diameter. Here we present three methods that minimize the displacement of analytes during matrix deposition, including a method where image resolution is not limited by t ...
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