实验仪器使用技术

This protocol provides an overview for performing a complete quantitative trait loci mapping experiment using the zebrafish, with an aim to use it to dissect a behavioral trait. This protocol covers the selection of the behavioral assay, the breeding design of the experiment, the generation ...

Zebrafish is rapidly becoming a popular model species for neurobehavioral and psychopharmacological research. The startle response represents the instinctive, evolutionarily conserved reaction of an organism to novel unexpected and/or aversive stimuli. While startle t ...

Although adult zebrafish are increasingly utilized as a model organism in neurobehavioral research, their habituation responses have only recently been evaluated in detail. When exposed to a novel environment, zebrafish demonstrate marked habituation responses, similar to t ...

Active avoidance conditioning in goldfish is a well-established behavioral paradigm to study learning and memory. Using active avoidance conditioning, investigators have discovered a good deal about learning and memory in goldfish. The behavioral methods and findings with gol ...

This protocol describes the cued fear conditioning task, designed to test the acquisition of the association between a fearful stimulus (unconditioned stimulus, US) and a cue (conditioned stimulus, CS) in zebrafish (Danio rerio). Here we use electrical shock as a US, and red light as a CS, both of whi ...

A brief summary of teleost spatial navigation research is followed by a description of a paradigm for the evaluation of egocentric-based, or viewpoint-dependent, spatial learning and memory function in zebrafish. The three-axis maze and testing protocol are used to evaluate the effects ...

Zebrafish are a popular model organism in neuroscience research, recently emerging as an excellent species to study complex social phenotypes. For example, zebrafish actively form shoals, which can be used to quantify their shoaling behaviors. Zebrafish also display strong social p ...

Zebrafish spend the majority of their time in groups, called shoals. Shoaling behavior is complex and dynamic: fish leave and rejoin the shoal, distances between shoal-members oscillate, and the speed and polarization of the shoal change on timescales of seconds to minutes. All these featur ...

In this protocol, we describe a high-throughput and inexpensive assay for anxiety-related behaviors in zebrafish. The behavioral observations occur while fish are housed individually in 1-L tanks commonly found in commercial zebrafish rearing systems. Behavioral indices incl ...

The Ca2+/calmodulin (CaM)-dependent protein kinase II (CaMKII) is a major regulator of synaptic �plasticity. CaMKII function depends on complex regulation of its activity and localization by Ca2+/CaM and several auto-phosphorylation reactions. Auto-phosphorylation at T286 ...

PKNs are serine/threonine protein kinases that have conserved catalytic domains homologous to those of protein kinase C (PKC) family members and regulatory regions containing antiparallel coiled-coil (ACC) domains and C2-like domains. PKN1 in particular is abundant in the brain, a ...

CASK, a scaffolding protein present in neuronal synapses and other cell junctions, contains a CaM-kinase domain at its N terminus. Due to lack of adequate biochemical data and based on bioinformatics, this domain was classified as a pseudokinase. But new evidence suggests that CASK is a unique ki ...

A major axis of inter-individual behavioral variation is correlated behavioral traits, known variously as behavioral syndromes, personality, or temperament. The zebrafish is a good model organism with which to explore the molecular genetic mechanisms of personality. The first st ...

The zebrafish model is being increasingly used in stress and anxiety research. The homologue of the hypothalamic-pituitary-adrenal (HPA) axis has been found in fish and is referred to as the hypothalamic-pituitary-�interrenal (HPI) axis. HPA (mammalian) and HPI (fish) axes are homolog ...

A primary goal in neuroscience is to understand how the brain gives rise to innate and learned behavior. One way to expand the contributions of zebrafish in this respect is to use a computerized system that can train larval zebrafish in various behavior assays. This will allow more efficient genetic ...

The study of diseases of the nervous system relies heavily on the use of animal models which try to replicate the human condition via various methods. The underlying assumption is that the root causes of the impairment are similar in the animal model and the human patient, thus validating the findings ...

Nerve injury and/or disease are known to provoke the release of various neurotrophic and inflammatory agents from spinal microglia and astrocytes. Prolonged exposure of spinal neurons to these ‘nociceptive mediators’ invokes pathophysiological changes that lead to the ‘centr ...

Artificially perfused brainstem preparations have several advantages over both in vivo and superfused en bloc preparations for studying respiratory circuits. They allow the analysis of neuronal function without confounding influences of anesthesia and major peripheral f ...

Neuronal networks can be properly analyzed in experimental preparations that preserve the largest �connectivity within and between brain structures. The isolated whole guinea pig brain maintained in vitro by arterial perfusion is ideal for this purpose. The methods and techniques ...

Rises of cytosolic Ca2+ (Cai) associated with early network oscillations (ENOs) are important for brain maturation. Thus, developing neural networks are often studied by combining Cai imaging with electrophysiological recording of extracellular activity and/or intracellu ...