Electrophysiological Technique for Analysis of Synaptic Function of PKN1 in Hippocampus

PKNs are serine/threonine protein kinases that have conserved catalytic domains homologous to those of protein kinase C (PKC) family members and regulatory regions containing antiparallel coiled-coil (ACC) domains and C2-like domains. PKN1 in particular is abundant in the brain, and the physiological role of this enzyme has been examined by generating PKN1 genetically modified mice and inhibitors of this enzyme. Here, we review electrophysiological techniques to analyze synaptic functions in the CA1 region of the hippocampus using these mice and inhibitors.