实验步骤 为了检测小胶质细胞的吞噬功能，利用凋亡的神经祖细胞作为目标，因为在体外环境下它能最模拟自然条件下小胶质细胞的吞噬目标。1. 将神经祖细胞（NPC）置于0.1M PBS 2mg/ml木瓜蛋白酶（Worthington）溶液中进行分离，37ºC作用30min。2. 将分离的NPC 在紫外光下处理15-20min。注：此步应该在一个很薄的塑料培养皿中进行，厚的塑料（如15或50毫升锥形管）将 ...
实验试剂 1. Absolute ethanol (96%-100%) 实验设备 1. Nuclease-free 50 ml centrifuge tube2. Water bath incubator or heating block preset at 65°C 3. Magnetic separation device for 50 ml tubes 实验步骤 1 ...
实验原理 E.Z.N.A.® SQ Tissue RNA uses a highly efficient solution based system to provide a convenient fast reliable and non-toxic method to isolate high quality RNA from various samples. Samples are fir ...
实验试剂 1. Complete growth medium pre-warmed to 37℃2. Balanced salt solution such as Dulbecco’s Phosphate Buffered Saline (DPBS) containing no calcium magnesium or phenol red3. Dissociation reagent such ...
实验步骤 1. Measure the volume of sample and adjust the sample volume to 100ul with DEPC-Water and proceed to step 2.2. Add 350ul QVL Lysis buffer and mix by vortexing at maximum speed for 15 seconds. Wh ...
实验原理 1. 单向琼脂扩散试验是一种定量试验，将抗体混合于琼脂内，倾注于玻片或平皿上，凝固后在琼脂上打孔，再将抗原标本加入孔内，经过一定的时间，在孔的周围出现抗原抗体复合物形成的沉淀环，环的大小与抗原含量和扩散时间相关。用不同浓度的抗原制成标准曲线，则未知标本中的抗原含量即可从准标曲线中求出。2. 抗原和抗体加到琼脂板上相对应的孔中，两者各自向四周扩散，如两者相对应，浓度比例合适，则经一定时间 ...
实验材料 NameCompanyCatalog NumberCommentsSodium chlorideSigmaS7653PBSPotassium chlorideSigmaP9333PBSSodium phosphate dibasicSigmaS7907PBSPotassium phosphate monobasicSigmaP5655PBSGlucoseSigmaG5400Isolat ...
实验步骤 The following protocol used formalin-fixed paraffin-embeddedhuman tonsil tissue (supplied by the Alfred Hospital Australia).1.Deparaffinize and rehydrate the tissue section. 2.Perform heat-ind ...
实验试剂 1. 细胞裂解液：10 mmol／L Tris-HCL(pH 8.0)，150mmol／LNaCl，10mmol／LEDTA，0.4％SDS，蛋白酶K 100ug／m1。2. RNase(10 mg／m1)：RNase溶解于100 mmol／LTris-HCl(pH7.5)及15 mmol／L NaCl中。煮沸15 min后缓慢冷却至室温，-20℃储存。3. TE缓冲液：Tris-HC ...
实验试剂 碱性磷酸酶缓冲液：100mmol／L NaCl，5mmol／L MgCl2，100mmol／L Tris(pH9.5)；NBT溶液(0.5gNBT用10ml 70％DMF溶解，贮存在4℃)；BCIP溶液(0.5gBCIP用10ml100％DMF溶解，贮存在4℃)； 20mmol／LEDTA用PBS溶解，DPX。 实验设备 光学显微镜(通常带有照相机以便于记录结果) ...
实验材料 1. 动物 6-10周的CBA/J小鼠 2. 寄生虫 鼠弓形虫 RH株速殖子 76K株囊肿 实验步骤 1. 弓形虫属超声处理的准备清洗速殖子，超声处理，离心。最后一次离心的上清液作为抗原的来源。蛋白浓度用蛋白试剂盒检测，以牛血清蛋白(BSA)作为标准。等分装好存于-80度备用。2. DC细胞系和外质体分离SRDC DC系SRDC是脾细胞DC系，形态、表型和活性与 ...
实验试剂 X Dulbecco’s Phosphate-buffered saline (D-PBS)Fixative solution: 4% Formaldehyde solution in PBS pH 7.4Permeabilizing solution: 0.25% Triton® X-100 in PBS pH 7.4Blocking solution: 5% Normal Goat ...
实验材料 NameCompanyCatalog NumberCommentsJumpStart REDTaq ReadyMixSigma-AldrichP0982GanciclovirSigma-AldrichG2536G418Sigma-AldrichG8168PuromycinSigma-AldrichP9620Topo TAInvitrogenKNM4500-01 GenElu ...
实验步骤 1. Specimen preparation: Golgi-Cox 1) This protocol closely follows the protocol of Mayerich et al.7. 2) The C57BL/6J mouse is deeply anesthetized using isoflurane inhalant anest ...
实验试剂 Regents supplied by user1. Ethanol -approximately 0.3 ml per sample. 实验设备 Equipments supplied by user1. Tabletop microcentrifuge and sterile 1.5 ml tubes2. Vacuum Manifold3. Water bath - se ...
实验试剂 人类胚胎肾（HEK）293T细胞腺病毒转移载体质粒腺病毒包装载体PLP1，pLP2（之前），PLP / VSVGDMEM培养基青霉素，链霉素-谷氨酰胺（100X）磷酸盐缓冲液PBS（-） 胎牛血清凝聚胺 实验设备 10-cm的细胞培养皿50毫升锥形离心管Steriflip-GP过滤器（0.22微米） 超离心管17.0mL5％二氧化碳培养箱荧光显微镜生物安全柜离心机 ...
实验原理 A mixture of mouse IgG antibodies against the non-CD4 T cells is added to the starting sample and allowed to bind to the cells. Depletion MyOne™ Dynabeads® are added and will bind to the antibo ...
实验原理 酵母细胞富含蛋白质和核酸。用稀碱液（0.2% 的氢氧化钠）处理酵母使细胞裂解，离心收集上清液，得到酵母核蛋白抽提液。用盐酸调节抽提液 pH 至 3.0 （核蛋白的等电点），核蛋白溶解度下降大量沉出，离心收集沉淀物为酵母蛋白质粗制品。酵母核蛋白是一种结合蛋白质，是蛋白质与核酸的复合物。酵母核酸主要是 RNA （含量为干菌体的 2.67 ％-10.0 ％）， DNA 含量较少，仅为 0.0 ...
实验步骤 1.Inoculate 2 ml of L2 media containing the appropriate antibiotic with a single bacterial colony picked using a sterile toothpick. Incubate at 37 degrees overnight with vigorous shaking.2.Tra ...
实验试剂 1.4 Male S. purpuratus sea urchins. (It is impossible to tell what sex sea urchins are until they shed gametes from the five pores on the top center of the animal thus order twice as many anima ...
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