生物信息学技术

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes a method to construct oligonucleotide conjugates labeled with 1,4,7,10?tetraazacyclododecane?1,4,7,10?tetraacetic

Abstract Table of Contents Figures Literature Cited Abstract Glide is a ligand docking program for predicting protein?ligand binding modes and ranking ligands via high?throughput virtual screening. Glide utilizes two different scoring func

Abstract Table of Contents Materials Figures Literature Cited Abstract ChIP?chip combines chromatin immunoprecipitation (ChIP) with microarrays (chip) to determine protein?DNA interactions occurring in li

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes a system for the automated high?throughput analysis of cell cytotoxicity in 96?well and 384?well microplates. Dis

Abstract Table of Contents Materials Figures Literature Cited Abstract Chromosome conformation capture (3C) is used to quantify physical DNA contacts in vivo at high resolution. 3C was first used in yeast

Abstract Table of Contents Materials Figures Literature Cited Abstract Peptide?oligonucleotide conjugates incorporating all the nucleobases and trifunctional amino acids are obtained by Diels?Alder reacti

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes a method allowing RNA visualization in live cells. The method is based on fluorescent protein complementation reg

Abstract Table of Contents Materials Figures Literature Cited Abstract SPOT arrays consist of synthesized peptides 12? to 18?amino acids long, with overlapping sequences that cover the entire sequence of

Abstract Table of Contents Materials Figures Literature Cited Abstract The detailed synthetic protocol for a 4?selenothymidine phosphoramidite and its use to prepare modified oligonucleotides is described

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit addresses the applications of fluorescence microscopy and quantitative imaging to study multiple physiological variables of

Abstract Table of Contents Materials Figures Literature Cited Abstract The classical yeast two?hybrid system and its modifications have been successfully used over the past decade to investigate interacti

Abstract Table of Contents Figures Literature Cited Abstract The Molecular Interactions Database (MINT) is a relational database designed to store information about protein interactions. Expert curators extract the relevant information fro

Abstract Table of Contents Figures Literature Cited Abstract This unit offers a general overview of several techniques that have been developed for inferring functional and/or protein?protein interaction networks. The majority of these use

Abstract Table of Contents Materials Figures Literature Cited Abstract Fluorescence resonance energy transfer (FRET) is a fluorescence microscopy technique suitable for live cells and capable of detecting

Abstract Table of Contents Materials Figures Literature Cited Abstract Investigation of single RNA molecules using fluorescence resonance energy transfer (FRET) is a powerful approach to investigate dynam

Abstract Table of Contents Materials Figures Literature Cited Abstract Labeling nucleic acids with radioisotopes, fluorophores, biotin, or digoxigenin enables their detection and analysis. When designing

Abstract Table of Contents Materials Figures Literature Cited Abstract Native gels enable the analysis of protein complexes on a proteome?wide scale in a single experiment. The proto

Abstract Table of Contents Materials Figures Literature Cited Abstract The Cu(I)?catalyzed alkyne?azide cycloaddition (CuAAC) reaction has been used to synthesize cyclic mini?DNA duplexes. The reaction is

Abstract Table of Contents Materials Figures Literature Cited Abstract The sequential functionalization of long?chain alkylamine controlled?pore glass (CPG) with a 3?hydroxypropyl?(2?cyanoethyl)thiophosphor

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes an acceptor?sensitized emission FRET method using a confocal microscope for image acquisition. In contrast to acc