实验动物技术

Gametes are collected as described. To remove some of their jelly coatwash eggs several times in ASW by allowing them to settle and gently pouring off excess liquid. Transfer enough eggs to barely cov ...

1. Transfer 50 ml of embryo cultures to centrifuge tubes. Spin at 1500 rpm for 5 minutes. Check that you can see a pellet of embryos at the bottom.Quickly pour off the ASW. Try to remove as much as po ...

Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity1. Obtain embryo samples tube of AP substrate buffer and tube of phosphate buffered saline (PBS) for each grou ...

1. Transfer fixed embryos to microfuge tubes. Allow to settle for 10 minutes.Gently remove most of the liquid.2. Add 100 ul antibody to one tube and 100 ul 1% normal goat serum to the other as a contr ...

The following is written to help non-marine biologists use sea urchins occasionally in teaching; disregard if you have running sea water tanks in your lab!Gravid animals can be obtained virtually year ...

Solutions for Sea UrchinASW: Artificial sea water (MBL)NaCl24.72 gKCl0.67 gCaCl2.2H2O1.36 gMgCl2.6H2O4.66 gMgSO4.7H2O6.29 gNaHCO30.18 gdH2Oto 1 literSulfate-free Sea waterNaCl27.72 gKCl0.67 gCaCl2.2H2 ...

Invertebrate embryos develop at different rates depending on the temperature of incubation. Descriptive stages based on the number of cells and their arrangement in the embryo are used to enable resea ...

Jill K. Flemming Franklin & Marshall College Class of 2001 IntroductionThe objective of this project is to observe the effects of UV radiation on both fertilization and development in the sea urchin e ...

Objective:To test the effects of UV radiation at 254nm on Sea Urchin embryo development after radiation at the 2-cell stage.Background:Sea Urchins exhibit radial holoblastic cleavage. The first and se ...

Objective: to test the effects of inhibiting the fast and slow blocks to polyspermy on sea urchin development. BackgroundSea urchin eggs release components of the egg jelly that attract sperm and indu ...

The Effects of NiCl2on Spicule FormationJessica Ann Billet Franklin and Marshall Class of 2000Background and ObjectiveSea urchins exhibit radial holoblastic cleavage eventually forming a blastula. Sho ...

ObjectiveTo observe the role sulfate plays in sea urchin gastrulation and to replicate the findings of Karp and Solursh that sea urchin embryos fail to gastrulate without sulfate. To test whether endo ...

ObjectiveThe purpose of this experiment is to determine the effects of calcium-free seawater on the development of sea urchin embryos. Embryos were placed in calcium-free sea after fertilization and a ...

Objective: to separate the Blastomere at different points in development and observe the growth of the resulting embryos. Procedure: We extractrf the gametes from the sea urchins fertilized the sea ur ...

Objective: This experiment aims to discover if temperature has an effect on the rates of sea urchin embryonic development. By altering the external environment the experiment will determine if tempera ...

Zebrafish will mate and deposit fertilized eggs on the bottom of the tank at 'dawn'. They can be accustomed to lay at any convenient time by keeping the room dark and lighting the tank with a timer. T ...

In order to repeat a published experiment or have someone else repeat yours it is important to use the same materials. For developmental studies that means knowing the precise point in development tha ...

AbstractPrevious studies by Stachel and colleagues indicate that lithium chloride induction of post-midblastular Brachydanio rerio embryos results in deficiencies in normal anterior-posterior developm ...

Objective The objective of the experiment is to determine the effects of ethanol exposure on the embryonic development of zebrafish through observation of physical defomities.IntroductionEmbryonic dev ...

IntroductionZebrafish have transparentembryos that develop outside the mother. They develop rapidly so that at 24 hours after fertilization the embryo has formed most of its tissue and organ primordia ...